文档标签: ab118970 Lipid Peroxidation MDA Assay Kit Protocol 系统标签: mda peroxidation assay lipid kit protocol ab118970LipidPeroxidation(MDA)AssayKitInstructionsrapid,sensitiveaccuratemeasurementLipidPeroxidationvarioussamples.researchuseonlydiagnosticuse.ProtocolSummaryAssayProtocolDataAnalysisTroubleshooting11Overview...
All the luciferase assays were perfomed using a Dual-luciferase assay kit according to the er’s protocol (Promega). HEK293T cells and Mouse embryonic fibroblasts (MEFs) were transfected with the indicated plasmids and reporter plasmids. To deplete TRIM25 from HEK293T cells, pSUPER.retro.puro...
and MDA5 dephosphorylation-mediated activation, we replaced hydrophobic and basic residues in the PP1-binding motif I or II of MDA5 and RIG-I with alanine; we then tested these mutants for their signal-transducing abilities in an IFN-β promoter assay (Figure 5A, lower panel; Figure S5A)....
Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage.These aldehydic secondary products of lipid peroxidation are generally accepted markers of oxidative stress.Catalog Number
Invasion assay. Cell migration experiments were performed in Boyden invasion chambers equipped with 8-mm pores filters and coated with Matrigel (Falcon, MA, USA). Equal volumes (0.5 ml per chamber) of cell suspension (3 Â 104 cells per ml) were used to seed the Matrigel invasion chambers...
Version:2LastUpdated:2January2014ab118970LipidPeroxidation(MDA)AssayKitInstructionsforUseFortherapid,sensitiveandaccuratemeasurementofLipidPeroxidationinvarioussamples.Thisproductisforresearchuseonlyandisnotintendedfordiagnosticuse.1TableofContents1.Overview32.ProtocolSummary43.ComponentsandStorage54.AssayProtocol75.Dat...
Wound healing assay For the wound healing assay, coordinates were marked on the 35-mm dish, and MDA-MB-231 cells were inoculated at a density of 1.5×106cells/well. When the confluence was ~100%, cells were rinsed twice with PBS, and serum-free DMEM was added for cell starvation. After...
2.2. MTS cell viability colorimetric assay For testing the effect of OLE and oleuropein on cell viability, cells were plated into 96-well microplates at a density of 5000 cells/cm2 for MDA-MB-468, 10,000 cells/cm2 for HCEpiC and MDA-MB-231 or 25,000 cells/cm2 for OVCAR-3 and OVCA...
Clinical utility of an enzyme-linked immunosorbent assay for detecting anti-melanoma differentiation-associated gene 5 autoantibodies PLoS One, 11 (4) (2016), Article e0154285 CrossrefView in ScopusGoogle Scholar [12] R.D. Sontheimer Dermatomyositis: an overview of recent progress with emphasis on ...
The present invention provides for assays for identifying such agents, which comprise combining, in an ATPase assay, MDA-5 protein and a test agent, measuring the ATPase activity of MDA-5 plus test agent, and comparing the resulting ATPase activity to the ATPase activity of the same amount of...