Cells (2 × 105/3 mL) were plated on cover slips in a six-well plate. For the nuclear translocation of NF-κB40, the cells were treated with 5 μM FTY720 for 24 h with or without 20 nM tumor necrosis factor-α (TNF-α) for 30 min. The cells were fixed in 2%...
– The type plate is located at the bottom of the system. – The voltage selector located at the bottom of this system is preset at 220V-240V from the factory. For 1 2 countries that operate at 110V-127V, For MCD700 please adjust to 110V-127V before you switch on the system. – ...
After that, tumorspheres were re-suspended into scat- tered single cells and reseeded into 6-well ultralow adherent cell culture plate (cell number in each well was kept in 5000). Five days later, the sphere number in each well was detected (second generation). Re- peated the re-...
After incubation, the bacterial colonies were treated with a 0.03% (w/v) Sudan Black B solution, which was evenly spread across the plate. The plates were then allowed to stand undisturbed at room temperature for 30 min. Subsequently, 96% ethanol was used to rinse off any excess staining ...