MCF-7 cells are fairly large adherent cells, with typical cell size measuring 20-25 microns. The recommended medium is EMEM containing 2 mM L-glutamine, 0.01 mg/mL bovine insulin (90%), fetal bovine serum (10%) and Earle’s BSS containing 1.5 g/L sodium bicarbonate, 0.1 mM non-essentia...
Doubling Time of MCF7 and SK-BR-3 cells in presence of different siRNAs on the CBC-1 (% of control).Joie N. MarhefkaRula A. AbbudAntaki
Similarly, 3D cultures of MCF10A cells form spheroids with a hollow lumen26, introducing phenotypes that would not have been present in 2D cultures9. However, few studies compared gene expression of cell lines in 3D cultures to primary tumours. Given the costs of 3D cultures27, there is a ...
Growth of MCF7 cells is inhibited by tumor necrosis factor alpha (TNF alpha). Secretion of IGFBP's can be modulated by treatment with anti-estrogens. MCF7细胞Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Cata...
In this work we studied the effects of the lack of Bcl2 in MCF7 cells.Methods. The breast cancer cell line MCF7 (Bcl2-positive) and its derivative MCF7/50B (Bcl2-negative) were compared in terms of the level of p53 expression, doubling time and distribution of cells among the cycle ...
MCF7/D40 cells display a classic-multi-drug resistance phenotype with expression of P-glycoprotein, decreased drug accumulation relative to the parental line and reversal of drug accumulation and drug resistance by verapamil. MCF7/Mitox cells likewise display resistance to multiple drugs, but in ...
Subsequently, the selection was conducted using G418, and doubling time was calculated in the transformed and control cells. RT and real-time PCR were also performed to analyze the expression of epithelial and mesenchymal genes and those related to CSCs.Results: According to ...