保存条件:低温避光纯度规格:1x10(6)viable cells/ml 产品类别:有机化学品 "MCF-10A 人正常乳腺上皮细胞系 细胞背景资料:乳腺;上皮细胞;自发永生;女性 细胞形态:上皮细胞样 细胞常规培养传代流程(请严格遵照无菌操作):1)吸出原培养瓶中的培养基,PBS缓冲液润洗细胞两次,加2-3ml 0.25%胰酶进行消化细胞(注意把握...
3T3 J2细胞系相关产品:Henrietta Lacks cells细胞、RPMI 8402细胞、G-401细胞 SK MEL-28细胞系相关产品:Mv 1 Lu细胞、SK-ChA1细胞、RGCs细胞 B-cell Acute Lymphoblastic Leukemia-1细胞系相关产品:SMMC7721细胞、NCI-HUT-460细胞、LSC-1细胞 SO-Rb 50细胞系相关产品:293/EBNA细胞、H-1385细胞、RASMC细胞 ...
Abstract 2327: Pyruvate carboxylase rescues MCF10A-ras cell viability in extracellular matrix detached conditionsBreast cancer is the most diagnosed cancer in women, and metastasis accounts for 90% of cancer related deaths. In order to metastasize, cancer cells must survive in conditions where they ...
The cells express normal p53 [9, 10], they do not form colonies in soft agar, and they do not grow in immuno-compromised mice [11]. Several transformed lines have been derived from MCF-10A cells. The MCF-10AT line was created by forced expression of activated H-ras [12, 13]. ...
Both inhibitors elevated the levels of RECK mRNA in MDA-MB-231 cells but not so much in MCF7 cells (Fig. 3f). In MDA-MB-231, upregulation of RECK could be detected at the protein level as well (Fig. 3g), supporting the involvement of RAS-signaling in RECK-repression in this cell ...
A human cell line (MCF10A) originated from spontaneous immortalization of breast epithelial cells obtained from a patient with fibrocystic disease. MCF10A cells do not survive in vivo in immunodeficient mice. However, T24 c-Ha-ras oncogene-transfected MCF10A cells (MCF10AT) form small nodules ...
Ras levels were increased by 3.5-, 12.0-, and 9.8-fold in the 10AT, CA1h and CA1a cells, respectively, relative to the 10A cells. PI3K activity was elevated by 2.4- and 2.5-fold in the CA1h and CA1a cells, relative to 10A cells, respectively. CA1h and CA1a are having a low...
While MCF-10A Ha-ras cells showed an increased sensitivity, MCF-10A c-erbB-2 and MCF-10A HE cells exhibited a relative resistance to taxol and taxotere, with an approximately 3.5- to 6.5-fold higher IC50 as compared with MCF-10A cells suggesting that c-erbB-2 overexpression has a d...
Tumor-associated immune cells often lack immune effector activities, and instead they present protumoral functions. To understand how tumors promote this immunological switch, invasive and noninvasive breast cancer cell (BRC) lines were cocultured with a promonocytic cell line in a Matrigel-based 3D...
关键字: MCF 10A人正常乳腺上皮细胞;人正常乳腺上皮细胞;MCF 10A细胞;MCF 10A cells; 公司简介 广州弗尔博生物科技有限公司(Guangzhou Fu'erbo Biological Technology Co.,Ltd.),致力于肿瘤免疫药研发临床前CRO服务和科研转化服务的国家高新技术企业。为全国客户提供PDX肿瘤标本库、原代ATCC细胞及其它源细胞、动物实...