Use of human breast cancer MCF7 cell line and all experimental animals were approved by Xi’an Jiaotong University Institutional Biosafety Committee. Funding The authors gratefully acknowledge financial support from the Project of Natural Science Foundation of Shaanxi Province, China (2013K09-27) and ...
The abnormal mRNA expressed by MCF-7 cells is identical to the larger form of V2 receptor mRNA produced by small-cell carcinoma [19]. This mRNA contains the entire structure for intron 2 of the gene, and is apparently the product of incomplete splicing [2]. The introduction of a tag stop...
In the described studies, we have developed a variant of the MCF-7 cell line, M-ERd3/g8, for analysis of 17-β-estradiol (E 2)-action without direct DNA interaction by E 2-receptors. M-ERd3/g8 cells principally express the estrogen receptor α (ER) form ERΔ3 which, due to ...
(G) Representative microscopic images of MCF-7 cells in different groups. (H) CCK-8 detected the inhibition effect of MCC950 on cell viability. *P < 0.05;**P < 0. 01; *** P < 0.001.Scale bars, 50 μm. Full size image TNF-ɑ-induced mitochondrial dysfunction promoted ...
We aimed to identify the key cancer-related processes that differ between the cell line analyses (MCF7-vs-MCF10A) and analysis of TCGA clinical samples (LumA-vs-normal), to understand the adequacy of cell lines as models for LumA BC. Unlike past methods which directly considered the differenc...
Morphological changes in the MCF-7 cell line were observed during the development of TAM-R. Cells' morphology has changed significantly; Cells treated with 30 μM TAM lost their epithelial-like shape and became round. At 40 μM, the cells were aggregated, and the rate of growth was higher...
These cells form slowly growing tumors in ovariectomized athymic nude mice but respond mitogenically upon estrogenic supplementation. The in vivo growth of MCF7/LCC9 tumors is not affected by treatment with ICI 182,780. Although there is some evidence of tamoxifen stimulation of tumor growth, this...
Acta Pharmacol Sin 2008 Mar; 29 (3): 385–396 Full-length article Modeling resistance index of taxoids to MCF-7 cell lines using ANN together with electrotopological state descriptors1 Pei-pei DONG2,3, Yan-yan ZHANG2,3, Guang-bo GE2,3, Chun-zhi AI2,3, Yong LIU2, Ling YANG2,5, ...
cell line MCF-7. It is described the fold change of each specific lipid within each family mentioned above that has been found within these four cell lines. There are differences in the presence of many PC, PE, SM and Cer, where an increased fold increase is present once their presence ...
This study aimed to elucidate the molecular mechanisms by which NSDHL regulates the capacity of BCSCs in the ER + human breast cancer cell line, MCF-7. Methods NSDHL knockdown suppressed tumor spheroid formation in MCF-7 human breast cancer cells grown on ultralow-attachment plates. RNA ...