3D cultures are superior to 2D cultures because they retain elements lost in 2D cultures, such as complex cell polarity and lumen formation23. 3D cultures of MCF7 result in spheroidal microtissues with luminal-like morphology and express more breast-specific biomarkers compared to 2D cultures24,25...
and an MCP-1 neutralizing antibody was able to reduce macrophage infiltration, angiogenesis and tumor growth in nude mice harboring human breast tumor xenografts.6 The epithelial–mesenchymal transition (EMT) is a complex process involved in tumor invasion and metastasis that is regulated by a variety...
MCF-10AT cells grown in three-dimensional basement membrane culture form complex multi-acinar structures that produce a basement membrane but undergo delayed cell cycle arrest and have incomplete luminal development. MCF-10CA1a cells grown in three-dimensional basement membrane culture form large, hype...
Oncology The Role of GSK-3beta in MCF-7 Breast Cancer Cell Signaling and Drug Resistance EAST CAROLINA UNIVERSITY James McCubrey SokoloskyMelissa LynnGlycogen synthase kinase- 3beta (GSK-3beta) is well documented to participate in a complex array of critical cellular processes. This versatile ...
Fig. 3 The effect of POX silencing on PEPD-p53 complex formation in breast cancer MCF-7 cells. Western blot for PEPD, p53 and POX after immunoprecipitation (IP) of PEPD (a) and p53 (b) in homogenates of MCF-7WT cells and MCF-7iPOX cells cultured in DMEM without glutamine and trea...
Transcriptional network building To elucidate complex relationships among the regulators of EMT in our dataset, a custom transcriptional network was built using the results of the differential expression analysis, previously reported associations between genes and EMT, as well as previously reported ...
They act in signal transduction, apoptosis, DNA repair, and malignant transformation (47–51). 14-3-3ζ is one of the members of this family, and increased expression has been revealed in early stages of several cancers including but not limited to liver cancer, head and neck squamous cell...
This leads to the formation of death-inducing signalling complex (DISC) resulting in proteolytic activation of procaspase-3/7[25]. Stimulation with chemotherapeutic agents are reported to cause caspase-8 activation which directly activates caspase-3[22, 28] or in the case of MCF-7 cells, ...
We previously reported that EBP50 activates autoph- agy by stimulating Beclin-1, which triggers the autophagic core complex[2]. Hence, we further examined whether EBP50- mediated lysosomal degradation of c-Myc was dependent on Beclin-1. Silencing of Beclin-1 delayed this degradation in cells ...
JNK and p38 are apoptotic mediators involved in cytokine and stress-mediated apoptosis, whereas ERK kinase and NF-κB are mainly responsible for cell survival in response to mitogenic stimuli.14–16 The mechanisms by which p38 promotes apoptosis are complex and involve its ability to regulate transc...