Methods Chemicals. All chemicals (> 97% purity) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bacterial Culturing and Analytical Methods. Strains MB and 11a were cultured in defined min-
(St Louis, MO, USA). Recombinant human HGF and anti-CXCR4 monoclonal antibody (MAB-182) were from R&D Systems (Abingdon, UK). Anti-acetyl-histone H4, anti-histone H4, anti-heterochromatin protein 1γ (HP1γ) (clone 42s2), anti-phosphotyrosine (clone 4G10), anti-c-Src (clone GD11)...
TRF, α-tocopherol and the individual tocotrienols were obtained from the Palm Oil Research Institute of Malaysia (PORIM), Kuala Lumpur. Tamoxifen and 17-β estradiol were purchased from Sigma Chemical (St. Louis, MO). MDA-MB-435 cells (Price et al. 1990) were obtained from Janet Price (...
Other chemicals, of reagent grade or better, were from Sigma Chemical (St Louis, MO). IL-8 antibodies and ELISA assay kits were from R&D Systems (Minneapolis, MN). PAR-1–specific monoclonal antibodies WEDE-15 and ATAP-2 were obtained from Beckman Coulter (Fullerton, CA) and Santa Cruz ...
Methods Chemicals. All chemicals (> 97% purity) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bacterial Culturing and Analytical Methods. Strains MB and 11a were cultured in defined min- imal salts medium under anaerobic conditions as previously described6,20. Briefly, ...
MTT assay The effect of various concentrations of TNF-α (10, 20, 50 ng/mL) on cell proliferation was examined by the 3-(4,5-dimethyl-thiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT, Sigma, St. Louis, MO) assays. In brief, the cells were plated in 96-well plates and ...
YAC-1 Cells;背景说明:YAC-1源自Mo-MuLV诱导的A/Sn小鼠淋巴瘤。细胞对NK细胞的作用敏感,可用于NK细胞活性检测。鼠痘病毒阴性。;传代方法:1:2传代;生长特性:悬浮生长;形态特性:淋巴母细胞样;相关产品有:THLE-3细胞、Hu-P-T3细胞、THP 1细胞 HOC1 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;...
Division of Endocrinology Metabolism and Lipid Research Washington University School of Medicine Campus Box 8127 660 South Euclid Avenue St Louis MO 63110 USADiabetologiaCryer PE. (2006) Comment on: Nathan DM, Buse JB, Davidson MB et al (2006) Management of hyperglycaemia in type 2 diabetes: a...
HLA-DR molecules bear the MB and MT antigens: , Depts. of the Med. and Path. and the HHMI Lab at the Washington Univ. Sci. of Med., St. Louis, MO, Immunology Branch, NCI, Bethesda, MD, and the Histocompat. Testing Lab, Miss. Gen. Hosp., Boston, MA.Show moreShow less...
Quantitative assessment of phenylalanine (Phe), tyrosine (Tyr) (both from Cambridge Isotope Laboratories, Andover, MA, USA), 3,5-dihydroxyphenylglycine (Dpg) and 4-hydroxyphenylglycine (Hpg) (both from Sigma-Aldrich, St. Louis, MO, USA) was carried out by LC-MS/MS (Mallinckrodt Baker B....