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The fixed cells were incubated with mouse monoclonal anti-α6 integrin antibody (1:300, Abcam, Cambridge, UK) for 1.5 hours at 37°C. The secondary goat anti-mouse Alexa Fluor 488 (1:200, Invitrogen) was used similarly. The cells were stained with DAPI (Invitrogen) and mounted with ...
The quality of the DNA was examined on a 1% agarose gel, and the purity of DNA was measured by a Nanodrop 2000 spectrophotometer (Thermo Scientific, Wilmingto, DE). Using the BGISEQ 500 sequence platform, high-quality and pure genomic DNA was resequenced, generating 150 bp paired-end ...
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