这个效率问题应该是酵母接合效率的问题了。我当时研究的课题是酵母染色质沉默区HML和HMR基因调控机制,经常用到酵母接合型转换(Mating type switching)的实验技术,对常用酵母菌株之间的接合效率如数家珍,于是我滔滔不绝,然后又三言两语建议改用质粒文库转化,因为质粒转化效率是完全可以提高的,不同的酵母转化方法,其效率...
网络释义 1. 常用到酵母接合型转换 什么意思_英语mating... ... mating type of yeast 酵母接合型mating type switching交配型转换 ;常用到酵母接合型转换... dict.youdao.com|基于3个网页
Mating-type switching entails the choice of a template for the gene conversions of the expressed mating-type locus. In the fission yeast Schizosaccharomyces pombe, correct template choice requires two competing small recombination enhancers, SRE2 and SRE3, that function in the context of ...
Localization of ASH1 mRNA to the distal cortex of daughter but not mother cells at the end of anaphase is responsible for the two cells' differential mating-type switching during the subsequent cell cycle. This localization depends on ac... I.Gonzalez,SB.Buonomo,K.Nasmyth,... - 《Current ...
The pattern of mating-type switching in cell pedigrees of the fission yeast Schizosaccharomyces pombe is dictated by the inheritance of specific DNA chains... B Arcangioli,TD Copeland,AJ Klar - 《Molecular & Cellular Biology》 被引量: 76发表: 1994年 Sap1, a protein that binds to sequences ...
In contrast, the RAD51 and RAD52 genes are essential for homothallic mating-type switching. We propose that mating-type switching in RAD54 mutants occurs stochastically with a low probability, possibly reflecting different states of chromosomal structure. 展开 关键词:...
SHIH et al., 1996 Schizosaccharomyces pombe rad23 is allelic with swi10, a mating-type switching/radioresistance gene that shares sequence homology with human and mouse ERCC1. Gene 170: 113-117.Hang, H., D. N. Hager, L. Goriparthi, K. M. Hopkins, H. Shih et al., 1996 ...
pombe . Heterothallic strains in which mating-type switching is much reduced or absent arise from the homothallic wild type either through the irreversible loss of one of the silent storage cassettes (generating heterothallic stable minus strain h S ) or through a genetic change which has ...
The mechanisms of unidirectional mating type switching in S. trifoliorum are not understood. The present study, comparing DNA sequences in the DNA region (MAT locus) that controls mating and spore production, found four main mating type genes (MAT1-1-5, MAT1-1-1, MAT1-2-1 and MAT1-2...
18.The possibility of switching from one mating type to the other is probably open to all population.或许所有种群都有从一种交配类型演变到另一种交配类型的可能性。 相关短句/例句 cross-match交叉配型 1.The evaluation of feasibility of kidney transplantation without donor cross-match in unsensitized ...