Haasbroek MP, Craven M, Barnes I, Crampton BG (2014) Microsatellite and mating type primers for the maize and sorghum pathogen, Exserohilum turcicum. Australasian Plant Pathology 43: 577-581.Haasbrock, M.P.; Craven, M.; Barnes, I.; Crampton, B.G. Microsatellite and mating type primers ...
Primers derived from the regions flanking this idiomorph were used to clone the oppositemating typeand sequencing identified theMat 1-1idiomorph (Waalwijket al.2002b). TheMATidiomorphs ofM. graminicolaare similar in structure to those of other ascomycetes. Each is about 3kb in size and contai...
type-specific loci corresponded to pheromone receptor Such linking makes the handling and analysis of multigenic genes from the A1 and A2 chromosomes (Yockteng et al., DNA polymorphism data sets more convenient (Filatov, Table 2 Loci used in this study N Loci PCR primers Forward primers ...
To ascertain the presence or absence of MID and FUS1 (encoding the mt+-specific glycoprotein29) in the wild-type and mating type-reversed strains, a PCR-based method was applied using mating type-specific primers pairs. Total DNA was extracted from 125 mL of a 4-day-old culture of C...
Primer pair 4 was used for this analysis. (F) Wild-type (A30235) and irt1-T MATa cells (A30246) were induced to sporulate, and Pog1 binding was determined at the indicated times. (G–J) Relative histone H3 occupancy across the IRT1/IME1 locus in MATa haploid (A4841) and MATa/...
Primer sequences of markers were blasted to the whole genome sequence of ATCC90797 (JGI). Physical positions of markers were indicated as the start and end positions of the primers. (XLSX 12 kb) Additional file 5: Synteny comparison of the genomic regions of the A mating-type locus and ...
In our previous work, degenerate PCR and chromosome walking technologies were used to obtain one pheromone receptor gene and one pheromone precursor gene from Lentinula edodes. In this study, four pairs of specific primers were designed according to the
graminea and screening of its mating type alleles using previously designed primer sets. Using the multiplex PCR assay, a 435-bp band was consistently amplified from all P. graminea isolates; while a 1300-bp fragment or a 1150-bp fragment was only amplified from the isolates harboring MAT-1 ...
These sequences were then used to design idiomorph-specific PCR primer pairs, allowing us to efficiently identify the mating types of isolates, a crucial component of our research on the environmental and genetic factors underlying this mixed mating system.R. E. Marra...
A 2966-bp DNA region was missing in the MAT locus of the self-sterile S mating type. The over- lapping 13 pairs of PCR primers designed based on genome sequence were used to amplify DNA fragments from genomic DNA of strains G22 (self-fertile) and 06CWM-G27 (self-sterile, ...