Determination of enzyme activity: The enzymatic activity was determined by a continuous colorimetric method by hydrolysis of BApNA at 405 nm (ε = 9.1 ˆ 103 M´1¨ cm´1 pH = 9.0) [58]. The free enzyme activity was measured in a 50 mM¨ L´1 carbonate/bicarbonate buffer system ...
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