Matchmaker Gold Yeast Two-Hybrid System(630489)是Matchmaker Gold产品线中深受欢迎的一款产品。可以用于体内大规模的初步筛选蛋白质相互作用候选目标,系统中包含新的Y2H Gold酵母菌株、更加严密的筛选报告基因、简单易用的文库、高水平表达的载体...
Matchmaker Gold 酵母双杂交系统 Matchmaker Gold酵母双杂交系统是我们深受欢迎的Matchmaker产品线中研究蛋白—蛋白相互作用的一款产品。这得益于系统中所包含的新的Y2H Gold酵母菌株、更加严密的筛选报告基因、简单易用的文库、高水平表达的载体以及基于SMART技术的文库构建试剂盒。 Matchmaker酵母双杂交系统 在基于GAL4的Ma...
630489Matchmaker® Gold Yeast Two-Hybrid SystemEach* Complete GAL4-based two-hybrid system to detect protein interactions. Yeast strain Y2HGold facilitates the elimination of many false positives that arise when screening an activation domain (AD) fusion library, because it contains ...
第五步:将合成的cDNA和线性化的pGADT7-Rec共转化到上述Y1HGold bait菌株中构建文库,同 时利用AbA选择培养基进行筛选。 第六步:收集获得的阳性克隆,并用Matchmaker Insert Check PCR Mix 2对文库中的插入片段进行分析。 菌落PCR分析 在Y1H及Y2H筛选中,菌落PCR是分析bait菌株并对阳性克隆进行分类的简便、快速的方法。
Improving the yeast two-hybrid system with permutated fusions proteins: the Varicella Zoster Virus interactome p pBackground/p pYeast two-hybrid (Y2H) screens have been among the most powerful methods to detect and analyze protein-protein interactions. However, they......
Our Matchmaker Gold Yeast One-Hybrid Library Screening System provides a simple and efficient method for identifying and characterizing novel protein-DNA interactions. All Matchmaker Gold Systems use Aureobasidin A resistance (AbAr) as a stringent, highly selective, and easy-to-use reporter. This nove...
Low Backgrounds with Aureobasidin A SelectionClontech’s Matchmaker™ Gold Yeast One-Hybrid Library Screening System provides a simple and
Matchmaker Two-Hybrid System 3 User Manual (PT3247-1) Yeast Protocol Handbook (PT3024-1) Vector Information Packet Storage Conditions–70°C ,pACT2,pGAD -Gal4,。Y2HGold,AH109Y187,Gal4strain.Traditional,,。,Yeastmaker?2.Alternatively,。,BNN132(2 × 1.0)AH109S。- 1945Matchmaker3(PT3247 - 1...
Matchmaker Gold酵母双杂交系统是我们深受欢迎的Matchmaker产品线中研究蛋白—蛋白相互作用的一款产品。这得益于系统中所包含的新的Y2H Gold酵母菌株、更加严密的筛选报告基因、简单易用的文库、高水平表达的载体以及基于SMART技术的文库构建试剂盒。 Matchmaker酵母双杂交系统 在基于GAL4的Matchmaker酵母双杂研究中,bait蛋...
Matchmaker Two-Hybrid System 3 User Manual (PT3247-1) Yeast Protocol Handbook (PT3024-1) Vector Information Packet Storage Conditions–70°C ,pACT2,pGAD -Gal4,。Y2HGold,AH109Y187,Gal4strain.Traditional,,。,Yeastmaker?2.Alternatively,。,BNN132(2 × 1.0)AH109S。- 1945Matchmaker3(PT3247 - 1...