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HPEKs were seeded at 1000–2000 cells/well in 6-well plates (in triplicates) with 5 ml of EpiLife Medium (ThermoFisher Scientific, Zug, Switzerland) with no BPE and 75–85% of human keratinocyte growth supplements (HKGS, ThermoFisher Scientific). 80% of the medium was replaced every 3...