Cell subtypeMarkerLocalizationSpecies Pan-granulocytesCD11bSurfaceHuman and mouse CD13SurfaceHuman CD15SurfaceHuman CD16/32SurfaceMouse CD32SurfaceHuman CD33SurfaceHuman Mast cellsFceR1SurfaceKey phenotyping marker: Human and mouse CD117(c-kit)SurfaceKey phenotyping m...
Prior to culture, 86% of cells were immunoreactive for the monocytic marker CD14, slightly fewer for CD11b and CD64, < 10% expressed Fc RIα, rare cells were CD34 + ( < 0,1%), and none stained for CD1, CD33, c-Kit, and tryptase. After 2 wk of culture, there ...
After 4 weeks, the purity of bone marrow-derived mast cells was evaluated by measuring the percentage of FceRI- and cKit-positive and CD11b negative cells on an LSRII Fortessa flow cytometer (BD Biosciences). The following antibodies were used: PE-cyanine7-conjugated anti-mouse CD117/cKit ...
Impaired mast cell development and innate immunity in Mac-1 (CD11b/CD18, CR3)–deficient mice. J Immunol., 161 (1998), pp. 6463-6467 CrossrefView in ScopusGoogle Scholar 29 MM Hamawy, C Oliver, SE Mergenhagen, RP Siraganian Adherence of rat basophilic leukemia (RBL-2H3) cells to fib...
On comparison of these cells with immature leukemic mast cells and basophils, all of the latter expressed CD68, and variable fractions were also CD11b and CD64 positive. In order to further characterize the potential mast cell precursor, flow cytometric analysis of double staining cells was done ...
Activation of mucosal mast cells promotes inflammation-related colon cancer development through recruiting and modulating inflammatory CD11b(+)Gr1(+) cells. Cancer Lett. 364, 173–180 (2015). Article CAS PubMed Central Google Scholar Sibilano, R., Frossi, B. & Pucillo, C. E. Mast cell ...
In AG129 mice infected subcutaneously with DENV2 (PL046 or mouse-adapted D2S10), viral E and NS1 proteins are detected in F4/80+CD11b+ macrophages and CD11c+ dendritic cells in the spleen and other lymphoid tissues during the early phase of infection [56]. By inoculation of labeled DENV ...
In contrast toMyb-74 GFP mice,Myb-68 GFP mice showed GFP activity only in a very small percentage of LS-K progenitor cells and CD11b+Gr1-myeloid cells (Fig.2aand Supplementary Figs.3a,b). Further analysis of LS-K progenitor cells demonstrated that GFP activity was found only in subsets ...
Prior to culture, 86% of cells were immunoreactive for the monocytic marker CD14, slightly fewer for CD11b and CD64, < 10% expressed Fc RIα, rare cells were CD34 + ( < 0,1%), and none stained for CD1, CD33, c-Kit, and tryptase. After 2 wk of culture, there was expression ...
A comparison of K5-R1/R2 and K5-R1/R2–CreMaster mice revealed no difference in the immune cell composition in the epidermis (Langerhans cells, αβ T cells, and γδ T cells) (Figure 6A) or in immune cell activation as determined by the analysis of CD69, CD86, and major ...