Flow cytometry is particularly useful in the diagnosis of systemic mastocytosis, where the mast cells may still occur at a very low frequency in the bone marrow, despite neoplastic growth. Numerous studies have compared immunophenotype of mast cells in mastocytosis and those in normal and diseased...
Request FREE Flow Cytometry Panel Design Help Mast cell development The development of mast cells remain poorly understood, as they are unique among hematopoietic cells in that they mature in peripheral tissues, rather than in lymphoid organs or in circulati...
Clear identification of specific cell populations by flow cytometry is important to understand functional roles. A well-defined flow cytometry panel for my... YRA Yu,DF Hotten,Y Malakhau,... - 《American Journal of Respiratory Cell & Molecular Biology》 被引量: 43发表: 2015年 Characterization...
Intestinal mast cells were analyzed using flow cytometry. (b) IL-4Ra is required for maximal mast cell expansion. Adoptive transfer of OVA-allergic cells into wild-type/IL-4Ra À / À mixed bone marrow chimeras (n ¼ 4–5 per group) favors the outgrowth of IL-4Ra-sufficient mast ...
Mast cell ZnT2 is required for normal wound healing Given the defective Zn release fromZnT2−/−BMMCs, we next examined the role of Zn localized in intracellular granules in a mast cell-mediated inflammatory process using mutant mice. Wound healing is a highly ordered and well-coordinated ...
After washing with PBS, the cell fluorescence was measured using a FACSCanto II flow cytometer (BD Biosciences). 2.5. Reconstitution of mast cells in mast cell-deficient mice BMMCs were prepared as previously described [13]. In brief, femoral bone marrow cells were harvested from 4- to 8-...
In the present study, we found expression of IL-3 receptors (IL-3R) in freshlyisolated human intestinal mast cells by reverse transcriptase (RT)-PCR and in mast cells cultured with stem cell factor (SCF) using RT-PCR and flow cytometry. IL-3R expression was enhanced when theculture ...
Functional aspects of mast cell-neuronal interactions remain poorly understood. Mast cell activation and degranulation can result in the release of powerful pro-inflammatory mediators such as histamine and cytokines. Cerebral dural mast cells have been p
Protease activated receptor 2 (PAR2) expressed by fibroblasts may be activated by serine proteases, such as the mast cell mediator tryptase. The objective in this study was to investigate the effects of mast cells and specifically mast cell tryptase on fibroblast migration and the role of PAR2 ...
Flow cytometry and immunofluorescence staining data showed that synovial mast cells in RA patients rarely expressed the proliferation marker Ki67 (Fig.1e, f). Contrastingly, the frequency of mast cell progenitors (MCP), reported as lineage-(CD4-CD8-CD14-CD19-) CD34hiCD117int/hiFcεRI+cells30...