Mast Cell Degranulation Assay KitOnly, U S EFor, N O TIn, U S EProcedures, Diagnostic
Streptavidin Magnetic Beads (P2151), BCA Protein Assay Kit (P0010), and Enhanced Chemiluminescence (ECL) Kit (P0018FM) were purchased from Beyotime (Shanghai, China). The mast cell degranulation assay kit (IMM001) was obtained from Sigma-Aldrich (Missouri, USA). Antibodies used in the ...
Our results indicate that NSP4 neither regulates the differentiation of GMPs to mast cells nor affects mast cell trafficking to tissues. In addition, NSP4 does not regulate mast cell degranulation, or expression of proinflammatory cytokines, chemokines, and proteases. However, loss of NSP4 significan...
Products Intro:Product Name:Mast Cell Degranulation Assay Kit CB Index:58 Related Information:Catalog(6672) Company Name: Beijing Xiangsheng Xingye Technology Co., Ltd. Tel:0136-81184382 13681184382 Products Intro:Product Name:Mast Cell Degranulation Assay Kit CB Index:58 Related Information:Catalog...
Mast Cell Degranulation Assay Kit 英文别名: Mast Cell Degranulation Assay Kit 中文名: 中文别名: CBNumber: CB45653139 分子式: 分子量: 0 MOL File: Mol file 化学性质安全信息用途供应商8 Mast Cell Degranulation Assay Kit化学性质 安全信息 Mast Cell Degranulation Assay Kit性质、用途与生产工艺 ...
Mast cells (MC) have been shown to mediate regulatory T-cell (Treg)-dependent, peripheral allograft tolerance in both skin and cardiac transplants. Furthermore, Treg have been implicated in mitigating IgE-mediated MC degranulation, establishing a dynamic, reciprocal relationship between MC and Treg ...
However, this type of assay cannot monitor degranulation of individual cells in real time, and it is not suitable for in vivo detection of degranulation. At the aim of real time imaging of mast cell degranulation at single cell level, we here developed a fluorescent protein-based indicator of...
The mast cell degranulation was verified by the amount of histamine released. Histamine was quantified in the supernatants obtained from the mast cells cultured alone after the various incubation time points. Histamine was measured by a sensitive radioenzyme assay based on the conversion of histamine ...
The next step was to investigate if the recombinant form of ArtinM would be able to induce mast cell degranulation. A dose–response curve for the release of β-hexosaminidase and TNF-α was performed (Fig. 3). In the absence of IgE, only the higher concentrations of rArtinM were able ...
Mast cell degranulation assay. BMMC's cultured for 4–6 weeks and seeded at 5 × 104 cells per well in HEPES buffer were analyzed for β- hexosaminidase enzyme release following exposure of either ENMs or dinitro- phenyl (DNP), as previously described40,59. Briefly, BMMC'...