Expression of a B-cell-restricted isoform of CD45 is associated with maturity in rat serosal and connective-tissue mast cellsAlcian bluearthritisconnective tissueratSafranin OserosalFas-associated protein with death domain/mediator of receptor induced toxicity (FADD/MORT1) was first described as a ...
The mast cell expansion in IL-4-treated wild-type and Il4raF709 mice occurred predominantly in the lamina propria, although a few mast cells were also observed in the villi and intestinal epithelium. Flow cytometry was used to quantify mast cells (CD45 þ lin À c-Kit þ IgE þ ...
a Synovial membranes from OA and RA patients were digested into single-cell suspension. Synovial mast cells were gated as CD45+CD117+FcεRI+ cells. Numbers indicated the percentages of mast cells in the gates. b Statistical analysis of mast cell percentage in synovial single-cell suspensions ...
Atkinson MA (2014) Losing a grip on the notion of β-cell speci- ficity for immune responses in type 1 diabetes: can we handle the truth? Diabetes 63:3572–3574 45. Orinska Z, Bulanova E, Budagian V, Metz M, Maurer M, Bulfone- Paus S (2005) TLR3-induced activation of mast ...
et al. Generation of Human Mesenchymal Stem Cell 3D Spheroids Using Low-binding Plates. Bio Protoc 8, e2968 (2018). Xu, C., Kim, N. G. & Gumbiner, B. M. Regulation of protein stability by GSK3 mediated phosphorylation. Cell Cycle 8, 4032–4039 (2009). Article CAS PubMed Google ...
All uveal mast cells were c-kit and CD45 positive and HLA-DR negative. No association existed between mast cells and actin-containing cells. Only a few mast cells were in close association with PGP 9.5-labeled nerve fibers. In the choroid, most mast cells were located in the inner central...
Mast cells are immune cells minimally present in normal tendon tissue. The increased abundance of mast cells in tendinopathy biopsies and at the sites of tendon injury suggests an unexplored role of this cell population in overuse tendon injuries. Mast c
At the same time, the increase in the concentration of MCs per 106 CD45 þ cells, peaks at D1 and persists on a plateau to D14 even though the inflammatory cell infiltrate, measured as number of leukocytes harvested, has returned to baseline. There are distinct differences in the level ...
(Skin mast cells were defined as CD117+CD45+ cells. A staining of FceRIa on skin mast cells was not reliable in our hands, likely due to loss of epitopes after enzymatic tissue digestion). In the five animals for which this mast cell population was investigated, the majority of the few...
The cells were then cultured in serum-free Iscove's methyl- cellulose medium (Stem Cell Technologies Inc.; Vancouver, BC, Canada) and Iscove's Modified Dulbecco's Medium (IMDM; Thermo Fisher Scientific; Waltham, MA, USA) supplemented with 200 ng/mL recombinant human stem cell factor (rhS...