MS/MS has been described as ‘taking the mass spectrum of an ion in a mass spectrum.’ The principle of MS/MS is that a sample is ionized and mass-analyzed in the first mass analyzer. A particular m/z value of interest is selected from the mass spectrum and is directed into a collis...
The proteins were precipitated in acetone at −80 °C for 1 h followed by centrifugation at 10,000 × g. The pellet was resuspended in 20 μl of 8 M urea with 10 mM DTT. After 30 min, iodoacetamide was added to a final concentration of 50 mM and the alkylation...
Ab initio study of multiphoton absorption properties of formaldehyde, acetaldehyde, and acetone. J Chem Phys, 1990, 92: 2495–2504 CrossRef Merchan M, Roos B O, McDiarmid R, et al. A combined theoretical and experimental determination of the electronic spectrum of acetone. J Chem Phys, ...
In the first row of the Library Search Parameters dialog box, click Browse and select the C:\DATABASE\NIST14.L folder (by default it is was initially C:\DATABASE\DEMO.L) and Click OK. Also, the Spectrum > Set Default Search Engine menu can be used to select whether to use the ...
The invention discloses a precise mass spectrum method to identify wheat low-molecular-weight glutelin subunit, which comprises: (1) to prepare the sample, using 35-45% acetone to deposit protein extract and centrifuge, selecting supernatant, and using 75-85% acetone to deposit the glutelin ...
Twelve micrograms of protein (0.6 μg/μl) was cross-linked with 1 mM ArGO in a buffer of 50 mM borate, 50 mM HEPES (pH 7.5) at RT for 1 h. The reaction was quenched using 5× volumes of acetone for at least 30 min at −20 °C to precipitate the protein....
The interpretation of the spectra was aided by metastable-ion peaks, mass spectra of D 2 O-exchanged analogs, and the mass spectrum of an O-isopropylidene derivative prepared with acetone-d 6 . 展开 DOI: 10.1080/00021369.1969.10859451 被引量: 10 ...
(FIG. 7). The samples are pooled and trypsinized together, and peptides comprising the affinity marker are recovered, e.g., using avidin-agarose and 2-ME, and simultaneously subjected to LC-ESI MS. Because each peptide has either a cysteineamide or a d3-cysteineamide, the MS spectrum ...
If z = 1, then ratio m/e is the relative atomic or molecular mass of the given ion. In contrast to spectroscopic methods, mass spectrometry does not utilize any clearly defined property of the molecule. Consequently, complete reproducibility of the mass spectrum of a pure substance cannot be...
What is more, the gas nature of n-butane is easy to control by changing flow rate; however, the generation of flame about liquid fuels, such as alcohol, ethyl acetate and acetone have to use cotton thread and the flame size is not easy to control. Considering the most important ...