至此,文章引言引出了本综述的第二和第三部分,也是文章的两大核心板块:即药物靶点鉴定和作用机制研究。 对于药物的靶点鉴定:则主要是化学蛋白质组学方法(chemical proteomics) ,该策略通过分析蛋白质的亲和力、活性、稳定性等来研究药物在体内细胞中的作用靶点。 一类是利用化学探针来识别蛋白质靶标的化学蛋白质组学。...
At the same time, recent developments in high-resolution mass spectrometry have provided the technology for searching the drug targets across the whole cell proteomes. This review provides a concise description of the main objectives and problems of mass spectrometry-based chemica...
The field of proteomics is built on technologies to analyze large numbers of proteins—ideally the entire proteome—in the same experiment. Mass spectrometry (MS) has been successfully used to characterize proteins in complex mixtures, but results so far
The field of proteomics is built on technologies to analyze large numbers of proteins—ideally the entire proteome—in the same experiment. Mass spectrometry (MS) has been successfully used to characterize proteins in complex mixtures, but results so far have largely been qualitative. Two recently d...
摘要: The term "chemical proteomics" refers to a research area at the interface of chemistry, biochemistry, and cell biology that focuses on studying the mechanism of action of bioactive small molecule comp关键词: Affinity chromatography Chemical proteomics Drug profiling Mass spectrometry Post...
News & Views24 Oct 2024Nature Chemical Biology Volume: 20, P: 1397-1398 Beyond protein lists: AI-assisted interpretation of proteomic investigations in the context of evolving scientific knowledge Mass spectrometry-based proteomics provides broad and quantitative detection of the proteome, but its resul...
In this review, we will focus on mass spectrometry based studies of virus proteins and virus–host interactions at the protein level. We will described the different MS methods used, followed by a survey of successful applications in virus proteomics. General techniques for mass spectrometry based ...
Mass spectrometry-based proteomics: current status and potential use in clinical chemistry - Binz, Hochstrasser, et al. - 2003 RD Binz - 《Clinical Chemistry & Laboratory Medicine Cclm》 被引量: 48发表: 2003年 Reproducibility in Protein Profiling by MALDI-TOF Mass Spectrometry BACKGROUND: Protein...
ofchallengestoproteinandproteomics researchers,andefficientandsensitive methodsfordetectionofPTMsare required.Traditionally,PTMshave beenidentifiedbyEdmandegradation, aminoacidanalysis,isotopiclabeling, orimmunochemistry.Withinrecent years,massspectrometry(MS)has ...
We review the fundamental principles of statistical experimental design, and their application to quantitative mass spectrometry-based proteomics. We focus... AA Oberg,OO Vitek 被引量: 225发表: 2009年 An Integrated Platform for Isolation, Processing, and Mass Spectrometry-based Proteomic Profiling of ...