An interesting feature of this derivatization procedure is that it distinguishes aspartic and glutamic acid from the respective amides. The structure of asparagine and glutamine derivatives has been established. A survey of oxazolidinone mass spectra has provided a list of diagnostically useful ions. ...
amino acidsThe chemical ionization mass spectra of fluorescamine and fluorescamine - amino acid derivatives have been studied using methane and ammonia as reagent gases. Major ions in the spectra are protonated molecular ions, adduct ions and ions formed by loss of an oxygen atom.doi:10.1080/...
During the preparation of the solutions, sterilized Eppendorf tubes (Eppendorf BIOPUR Safe-lock tubes) and pipet tips were used (Eppendorf BIOPUR epT.I.P.S.). To prepare the samples on the sample holder, 1 µL of an amino acid or salt solution is dropcast into a cavity and left to...
AntiSMASH46 revealed a non-ribosomal peptide (NRP) BGC in the genome of Streptomyces sp. NRRL B-5680, with seven adenylation domains that are highly specific to the amino acid residues of dinghupeptin molecules47 (Fig. 7a). After a BLAST search of this BGC against the NCBI nucleotide ...
The monoisotopic mass of an ion or molecule is defined as an exact mass and is determined by summing each mass of the most abundant isotope (e.g. 1H=1.00782, 12C=12.00000, 16O=15.99491, 14N=14.00307) present in the chemical formula. ...
It is well known that a high-protein diet contributes to an elevation of mixed MPS (Pasiakos et al. 2013) and whole-body protein synthesis (Gwin et al. 2020a) as well as helping to inhibit insulin-sensitive protein breakdown (insulin-IGF-1-PI3K), which therefore partially counteracts the...
The method allows the simultaneous quantification of pyrraline in addition to the common amino acids, as well as acid-labile components such as the Amadori products and tryptophan, at levels lower than 500 mu-g/kg protein. Values of ... T Henle,H Klostermeyer - 《Z.lebensm.unters.forsch》...
addition to intact characterization, you can confirm amino acid sequence, identify site and type of known/unknown post translational modifications, while providing relative amounts using Peptide Mapping workflows. Additional features include disulfide linkage, sequence variant analysis, and sequence ...
An "Averagine" model is used to describe the composition of an "average amino acid", which can then be used to approximate the composition and isotopic abundance of a combination of specific amino acids. Given that often the only solution available is to guess at the composition of a ...
Over the past decade, cross-linking mass spectrometry (CLMS) has developed into a robust and flexible tool that provides medium-resolution structural information. CLMS data provide a measure of the proximity of amino acid residues and thus offer informat