Mass Spectrometry of Natural Substances in Food The analysis and metabolic studies of amino acids, peptides, proteins, lipids, sugars, carbohydrates and vitamins is also discussed, with separate chapters ... F Mellon,JR Startin,R Self 被引量: 19发表: 2000年 High-performance liquid chromatography...
Label-free characterization of single biomolecules aims to complement fluorescence microscopy in situations where labeling compromises data interpretation, is technically challenging or even impossible. However, existing methods require the investigated
Identification of small molecules is a critical task in various areas of life science. Recent advances in mass spectrometry have enabled the collection of tandem mass spectra of small molecules from hundreds of thousands of environments. To identify whic
The fast atom bombardment mass spectra of 24 α‐amino acids have been studied. These include the mass spectra as well as the metastable ion MI and collisional activation CA spectra of [M + H]+ and [M ? H]? ions. The extent of the common neutral losses as NH3, H2O and CO2H2 in ...
Prenylation is a complex process that involves several enzymatic steps. The first step is the addition of an isoprenoid group to the cysteine residue of the target protein. This is followed by proteolytic cleavage of the terminal amino acids of the protein, which exposes the prenylated cysteineresi...
Although sulfolane proved unexpectedly to be a poor solvent for solution-phase secondaryion mass spectrometry of underivatized amino acids in the presence of thallium(I) salts, glycerol was somewhat more effective. Also, the addition of trifluoromethanesulfonic acid proved more effective than addition...
However, this approach is hampered by a wide range of preanalytical variables. Hence, the aim of our study was to develop a standardized preanalytical protocol for metabolite profiling of amino acids and acylcarnitines in human blood. Amino acids and acylcarnitines were simultaneous analyzed after ...
(Extended Data Fig.1a). Free C17:0 and d-C18:1 fatty acids had a similar decay profile, comparable to the endogenous oleic acid C18:1 (Extended Data Fig.1b). This decay was neither due to sequestration of fatty acids in phospholipids, as CFA-PE did not enrich over time (Extended ...
Different types (light to heavy) of laying hens are used in practice. There are questions about the optimum level of balanced protein (BP) supply in feed for different types of hens. Therefore, a broad range of amino acids intake levels [550 to 800 mg of true fecal digestible (TFD) Lys...
Using the condensation of N-protected amino acids with 2,6-bishydroxylmethyl pyridine, we synthesized six di-esters and four mono-esters. All ten esters are new compounds. They are all important intermediate compounds for synthesis of macrocylic compounds containing amino acid and pyridy...