Update from #4295 (comment): AFAIR Vim takes all local marks from the Vim instance if there is needed buffer and all local marks from viminfo if there is no, so delmarks works. But global marks are always merged, so it does not. So the fact that it works in Vim is an accident be...
To perform ChIP, small pieces of frozen testis tissue were cross-linked with 2% (w/v) formaldehyde at room temperature for 30 min using end-over-end tumbler. We next crushed fixed tissues in the presence of ChIP lysis buffer (1% (w/v) SDS, 10 mM EDTA, 50 mM Tris-HCl, pH 8...
Plants can produce organs throughout their entire life from pluripotent stem cells located at their growing tip, the shoot apical meristem (SAM). At the time of flowering, the SAM of Arabidopsis thaliana switches fate and starts producing flowers instead
Mann CD, Neal CP, Garcea G, Manson MM, Dennison AR, Berry DP: Prognostic molecular markers in hepatocellular carcinoma: A systematic review. Eur J Cancer. 2007 Google Scholar Nagao T, Inoue S, Yoshimi F, Sodeyama M, Omori Y, Mizuta T, Kawano N, Morioka Y: Postoperative recurrence of...
Briefly, 1 μL of Boc-Lys(crotonyl)-AMC stocking solution (10 mM) and 200 ng HDACs (1∼11) were added to the reaction buffer (25 mM Tris·HCl, 130 mM NaCl, 3.0 mM KCl, 1 mM MgCl2, 0.1% PEG8000, pH = 8.0) and incubated at 37°C for 3 hr. The reaction was then stopped...
All ChIP experiments were carried out on 2X107 cells per antibody. Cells were cross-linked with 1% formaldehyde, lyses and syndicated in sonication buffer (10 mM Tris–HCl pH 7.5, 200 mM NaCl, 1% SDS, 4% NP-40, 1 mM PMSF) to obtain an average chromatin size of 200-500 bp...
For DNMT1 immunostaining, unstained sections of prostate xenograft tumor tissue or human lung cancer tissue microarrays (purchased from US Biomax, Derwood, MD) were deparaffinized, hydrated, and subjected to heat-induced antigen retrieval using 40 mM sodium citrate buffer (pH 6.0). Staining was ...
of the four cell lines were separated by 1D SDS-PAGE under the following conditions: 2.5 µg protein load per 2.5 mm-wide lane of 1 mm-thick NuPAGE Novex bis-Tris 4–12% gels/format 26 well, run in triplicate in an XCell4 SureLock™ chamber filled with MES SDS running buffer, at...
Regarding HSV-1, notwithstanding the amount of data on the mechanisms regulating its latency and reactivation in neurons, the long-term effects of recurrent infection on aging are poorly characterized, and the possibility that this infection may also impair and accelerate the normal neuronal aging ...
iovec_base: // I/O config buffer for vmsplice(2) system call .zero 16 error_write_buffer: // I/O data buffer for write(2) system call .zero 1 .p2align 9,0 bytecode_storage: // the rest is a buffer for storing bytecode