Forward primer 5′- CAGAGCTTGCGAGAAAGG-3′ and reverse primer 5′- GGCGGAGGTGAACTTGTAG-3′ were used for genotyping PCR of RUBY. Phenotyping The fluorescence signals of eYGFPuv were visualized under a 365 nm wavelength UV light and imaged using an iPhone 11 as described by Yuan et al...
The primers used to detect the presence of the HPT gene were HP1 (5′-GCTTCTGCGGGCGATTTGTGT-3′) and HP2 (5′-GGTCGCGGAGGC TATGGATGC-3′), while primers INT-F (5′-CCTCGTAATCAATTGTTAGGAT-3′) and NOS-R (5′-GACCGGC AACAGGATTCAAT-3′) were used to detect the presence ...
AB455231. To construct the pBacIE1-NAT/3xP3EGFP plasmid, the IE1 portion of pIEx-2 (Novagen) was amplified with the primers hr5-IE1-BglII-s (50-CATTGCGTAGAT CTCGCGTAAAAC-30) and hr5-IE1-BlnI-a (50-AAAAAAAACCTAGGTCACTTG GTTGTTCACGATC-30) and ligated into the 6-kb portion ...
mGli1: Rev: 5′-CGGCTGACTGTGTAAGCAGA-3′ This paper N/A Software and algorithms ImageJ software Bethesda, MD N/A Imaris software (version 9.0.2) Bitplane N/A GraphPad Prism 7 GraphPad N/A MATLAB version R2019a MathWorks N/A IncuCyte software Sartorius N/A Other SP8-X confocal micr...
The primers of CXCL10 were 5’-GTACGCTGTACCTGCATCAGCATTAG-3’ and 5’-CTGGATTCAGACATCTCTTCTCACCC-3’. The relative abundances of the target genes were determined by standard curve and the GAPDH was set as loading control. Differential expression of genes and clinical characteristics SPSS ...
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We identified cancer patients in the Vstra Gtaland Region of Sweden diagnosed in 2011 with prostate, breast, colorectal, lung, gynaecological, and skin cancers including malignant melanoma. We studied the symptoms and diagnoses identified by diagnostic codes during a diagnostic interval of 12 months ...
The primers were as follows: NudCD1-for- ward, 5'-CCTGCTTCTGTTTGCGCCATG-3; NudCD1 -reverse, 5'-GAAGGCACTCACAAAGGGCTG -3'; Feng et al. BMC Cancer (2022) 22:981 Page 4 of 13 GAPDH-forward, 5'-ACAACTTTGGTATCG...
Genotyping for the polymorphic microsatellite repeat in the intron was performed using oligonucleotide pri- mer pairs 5¢-TTTGGCTGGTACAATCTCAATGG-3¢ and 5¢-TTCTGGGCACCCGTAACCTA-3¢. PCR was carried out in a total volume of 15 ll containing 25 ng of genomic DNA, 1.25 pmol of each...
IL-6 reverse, 5ʹ-ATGCCCATTAACAACAATCTGAGGTG-3ʹ; CCL5 forward, 5ʹ-CAGTCGTCTTTGTCACCCGA-3ʹ; CCL5 reverse, 5ʹ-GAG CAAGCAGAAACAGGOAAA-3ʹ; ribosomal protein L13a (RPL13A) forward, 5ʹ-CCTGGAGGAGAAGAGGAAAG-3ʹ; RPL13A reverse, 5ʹ- TTGAGGACCTCTGTGTATTT-3ʹ. ...