The reaction mixture was transformed into DH5α competent cells, and the colonies were selected at 37 °C. Individual colonies were picked, and plasmid DNA was isolated and verified by Sanger sequencing. The sequences of TSS-targeting gRNAs and controls used in Fig. 1 and Additional file 1:...
The reaction mixture was transformed into DH5α competent cells, and the colonies were selected at 37 °C. Individual colonies were picked, and plasmid DNA was isolated and verified by Sanger sequencing. The sequences of TSS-targeting gRNAs and controls used in Fig. 1 and Additional file 1:...
5). During printing, we scan the colonies, dispensing only ones falling within a narrow cell density range (see “Methods”)11. Fig. 3: Mapping g2ps1 mutant catalysis through metabolic biosensing. a Overview of the slide (75 mm × 25 mm) for single variant µMS comprising 10...
Student will create maps of the American colonies to reflect the economic activity and political institutions of the different colonial regions. They will also learn about the conflict between colonies over land and resources. Finally, students will explore the intersections of class and culture by ...
In addition, power requirements may vary between animals due to varying degrees of opsin expression in different colonies (Mattis et al., 2012). Thus, in each experimental session, we considered both individual differences in animals and potential long-term fluctuations in laser performance over ...
Each of the resulting fragments was randomly inserted into one of the three possible translation frames, followed immediately by a stop codon. (C) Plasmids from this library were transformed into ∼5 × 106 yeast colonies (Leu selection). These were harvested into a single flask and kept in...
Single cell-derived colonies were screened for bi-allelic deletion of the targeting region. A similar strategy with sgRNAs targeting flanking regions of selected enhancers has been applied to generate biallelic deletion of the late enhancer in Ddit4 locus, and biallelic deletion of the early ...
It is becoming evident that town planning, building, road programmes and the like make up-to-date maps a necessity and not a luxury. It is not to be wondered at that similar problems have arisen in the African Colonies, the budgets of which have been just as hard hit as that of ...
Social interactions powerfully impact the brain and the body, but high-resolution descriptions of these important physical interactions and their neural correlates are lacking. Currently, most studies rely on labor-intensive methods such as manual annotation. Scalable and objective tracking methods are re...