The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. ---质量投诉流程1、向我们的...
Immunohistochemical analysis of paraffin-embedded SNU1 xenograft, using MAP4K4(GTX116008) antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min GTX116008 WB Image Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ...
Membranes were washed with TBST and incubated with an appropriate secondary antibody for 1 h at room temperature. Proteins were visualized by enhanced chemiluminescence and quantified by densitometry. 2-Deoxyglucose Transport—Glucose uptake was performed as described previously for primary human muscle ...
MAP4K1HPK1HPK1KO mice show enhanced T-cell activation, increased cytokine production, and increased antibody production after KLH immunization. HPK1KO mice are more susceptible to EAE induction. HPK1KOT cells and B cells show enhanced cell activation and antigen receptor signaling. HPK1KO dendritic ce...
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Bound antibody was detected with a horseradish per- oxidase (HRP)-conjugated secondary antibody at room temperature for 1 hr (Cell Signaling Technology, MA, USA), and immunodetected proteins were visualized using the enhanced chemiluminescence (ECL) system. To confirm the equal amount of protein ...
Np-pep, non-phos-peptide; p-pep, phos-peptide; Np-Ab, non-phos-antibody; p-Ab, phos-antibody. (d) Confirmation of adenovirus transfection at comparable levels in cardiomyocytes. Total cell extracts from cardiomyocytes after transfecting MAP4, MAP4(Glu) or MAP4(Ala) adenovirus were analyzed...
Antibody 抗原 A synthetic phosphorylated peptide around T261 of human MAP2K4 (NP_0030011) 查看免疫原 偶联物 Unconjugated 形式 Liquid 浓度 0.75 mg/mL 纯化类型 Affinity chromatography 保存液 PBS, pH 7.3, with 50% glycerol 内含物 0.02% sodium azide 保存条件 -20°...
After blocking with BSA, the cells were incubated overnight with primary antibodies anti-MyHC (DF9647, Affinity, Changzhou, China), anti-LC3 (Cell Signaling Technology, Boston, USA) and anti-MAP4 (Abcam, London, UK) at 4 °C overnight. The primary antibody was washed out of the cells,...
HGK antibody was generated by immunization of rabbits with individual peptides (HGK epitope: HAPEPKPHYDPADRAREV). Anti-actin and anti-tubulin antibodies were purchased from Sigma. Anti-p-IRS-1 (S612; 2386), anti-p-IRS-1 (Y896; 3070) and anti-IRS-1 (#2382) antibodies were purchased ...