The depletion of disruptive variation caused by purifying natural selection (constraint) has been widely used to investigate protein-coding genes underlying human disorders1,2,3,4, but attempts to assess constr
2. FXS is the most common heritable cause of intellectual disability, as well as the largest single genetic contributor to autism spectrum disorder (ASD)3. In addition to characteristic executive function impairment, learning deficit, and social anxiety, about 50...