An Arabidopsis thaliana mutant that lacks a cambium and does not undergo secondary growth was identified. This mutant was called cambiumless and it was postulated that the CAMBIUMLESS protein was a positive regulator of vascular cambium development. A map-based cloning strategy was used to identify...
Map-based cloning has been widely used to identify genes responsible for mutant phenotypes in Arabidopsis, especially those mutants generated by EMS or fas... E Drenkard,BG Richter,S Rozen,... 被引量: 0发表: 2017年 A platform of high-density INDEL/CAPS markers for map-based cloning in Ar...
1.Morphological Research of Rice Root Hair Mutants by Cryo-SEM and Map-based Cloning of Mutant Genes;水稻根毛突变体Cryo-SEM观察及突变基因的图位克隆 2.The high-density markers are necessary for map-based cloning of rice genes,but the available marker could not reach the satisfying level.在水稻...
A homolog in Arabidopsis (AT4G27750) is involved in sugar sensing and carbohydrate synthesis, and mutants for the gene have restricted seed setting50. In rice, grain width generally differs between indica and japonica accessions, to the extent that this characteristic can be used to distinguish ...
Cloning and functional expression of the gene encoding the key enzyme for chlorophyll b biosynthesis (CAO) from Arabidopsis thaliana. Plant J. 2000;21(3):305–10. Harper AL, Gesjen SE, Linford AS, Peterson MP, Faircloth RS, Thissen MM, et al. Chlorophyllide a oxygenase mRNA and protein ...
Map-based cloning-new 图位法克隆基因 Genemap-basedcloning Genemap-basedcloning Firststep:Genemappedonthechromosome Pool-1(DNAmixtureof20individuals)Pool-2(DNAmixtureof20individuals)MarksonCH2 MarksonCH4MarksonCH3MarksonCH1 MarksonCH2MarksonCH4 MarksonCH5 MarksonCH3 MarksonCH1 MarksonCH5 Pool-1(...
Motif-based endomembrane trafficking. Plant Physiol. 186, 221–238 (2021). Article CAS PubMed PubMed Central Google Scholar Park, M. et al. Arabidopsisμ-adaptin subunit AP1M of adaptor protein complex 1 mediates late secretory and vacuolar traffic and is required for growth. Proc. Natl ...
Viruses are obligate intracellular parasites that have co-evolved with their hosts to establish an intricate network of protein–protein interactions. Here, we followed a high-throughput yeast two-hybrid screening to identify 378 novel protein–protein i
1998a. Molecular cloning and characterization of three cDNAs encoding putative mitogen-activated protein kinase kinases (MAPKKs) in Arabidopsis thaliana. DNA Res. 5: 341–348. PubMed Google Scholar Ichimura, K., Mizoguchi, T., Irie, K., Morris, P., Giraudat, J., Matsumoto, K. and ...
Cloning quantitative trait locus (QTL) is time consuming and laborious, which hinders the understanding of natural variation and genetic diversity. Here, we introduce RapMap, a method for rapid multi-QTL mapping by employing F2 gradient populations (F2GP