【解析】 图位克隆法(map-based cloning):根据功能基因组中都有相对较稳定的基因,在利用 分子标记技术对目标基因进行精细定位的基础上,用与目的基因紧密连锁的分子标记筛选 DNA文库(包括YAC,BAC、TAC、PAC和cosmid文库),从而构建目的基因区域的物理图谱, 再利用此物理图谐通过染色体步移逐渐逼近目的基因或染色体登陆(...
必应词典为您提供mapbasedcloning的释义,网络释义: 图位克隆;定位克隆;基因克隆;
Map-Based Cloning of PDV1 The pdv1-1 mutation was mapped with molecular markers based on a cleaved amplified polymorphic sequence (Konieczny and Ausubel, 1993) and simple sequence length polymorphisms (Bell and Ecker, 1994). We used some markers listed on The Arabidopsis Information Resource (T...
图位克隆法(map-based cloning)相关知识点: 试题来源: 解析 分离未知性状目的基因的一种方法。根据遗传连锁图,将目的基因定位到某个染色体的特定位点,并在其两侧确定遗传距离最近的分子标记。可以通过染色体步移,建立包含目的基因位点的多层次重叠群,最后经表型分析、功能互补等方法确定目的基因。 暂无解析...
Isolation of gene(s) on the basis of chromosome walking and propagation usually by YAC and/or cosmid clones. After a genome sequence has been completed, positional cloning will no longer be needed as...doi:10.1007/978-1-4020-6754-9_9837George P. RédeiSpringer Netherlands...
Map-basedcloning Map-basedgenecloning •Geneisolationwithoutpriorknowledgeofthegeneproductandusingthegeneticrelationshipbetweenageneandamarkerasthebasisforbeginningasearchforagene.•Chromosomewalking1.Movingtowardagenebyusingaprobeforamarkernearagene,toselectagenomicclonenearthegeneandmovingtowardthegene,by...
From Markers to Cloned Genes: Map-Based Cloningfrom markers to cloned genes: map-based cloningdoi:10.1007/3-540-26538-4_4W.-R. ScheibleMax-Planck-Institute of Molecular Plant PhysiologyO. TörjekUniversity of PotsdamT. AltmannUniversity of Potsdam...
Map-based cloning-new 图位法克隆基因 Genemap-basedcloning Genemap-basedcloning Firststep:Genemappedonthechromosome Pool-1(DNAmixtureof20individuals)Pool-2(DNAmixtureof20individuals)MarksonCH2 MarksonCH4MarksonCH3MarksonCH1 MarksonCH2MarksonCH4 MarksonCH5 MarksonCH3 MarksonCH1 MarksonCH5 Pool-1(...
问答题 图位克隆法(map-based cloning) 答案:分离未知性状目的基因的一种方法。根据遗传连锁图,将目的基因定位到某个染色体的特定位点,并在其两侧确定遗传距离最近的分子标... 点击查看完整答案手机看题 你可能感兴趣的试题 问答题 端粒酶(telomerase) 答案:端粒酶是一种特殊的DNA聚合酶,即自身携带RNA模板的逆转录...
各位大侠，谁能用比较通俗易懂的道理解释一下map-based cloning的原理和步骤呢？没做过这个实验，真是...