With SV40-based vectors, more than 550,000 glucocorticoid receptors (GRs) per cell could be obtained in COS-7 cells with good cell survival. In experiments with a CMV-driven vector expressing an enhanced Green Fluorescent Protein (EGFP), 54% of the cells were transfected, and 77% of EGFP ...
MAMMALIAN EXPRESSION VECTORS AND USES THEREOF The present invention features nucleic acids for recombinant protein expression in mammalian cell culture. The episomal vectors of the invention promote high protein production in mammalian cells expressing the SV40 T Ag or Epstein-Barr ... CM Hsieh 被引量...
We made several changes to the mMoClo plasmids originally described33 in order to incorporate them into the workflow for our laboratory, in which many constructs are prototyped using pcDNA-based expression vectors. Details can be found in Supplementary Fig. 9, Supplementary Data 1, 2. We modified...
The KRAB-EL222 module was cloned into an SV40 promoter-driven mammalian expression vector (pKM565) (Fig. 1a). As a light-regulated protein degradation system, we chose the B-LID system6. The functionality of both modules was assayed using a firefly luciferase (FLuc) reporter (pMZ1203), ...
(1982). Transformation of Mammalian Cells to Antibiotic Resistance with a Bacterial Gene Under Control of the SV40 Early Region Promoter. J. Molec. Appl. Gen. 1, 327-339. Wigler, M., Silverstein, S., Lee, L.-S., Pellicer...
An independent puromycin resistance cassette under an SV40 promoter was also included (Figure S10A in Supporting Information). We compared A-to-G editing efficiencies between the all-in-one and pooled Dvu-ABE vectors at ASGR1-2, HPRT-2, and HPRT-4 sites using Sanger sequencing and EditR ...
Each vector contains the neomycin/kanamycin-resistance gene under control of the prokaryotic β-lactamase promoter to provide kanamycin resistance in bacteria and the SV40 early promoter to provide G418 resistance in mammalian cells. pNTAP Expression Vectors The pNTAP expression vector is used to clone...
A detailed study by Hu and Davidson wherein lac operator sequences are inserted at different positions in the SV40 promoter region, indicates that in all cases there is a decrease in promoter activity due to the insertion per se [36]. More recently, Hedengren-Olcott and Hruby [37] have ...
e Translational regulation by MCP-based STIF and other state-of-the-art regulation strategies. For STIF-mediated translation (red), HEK-293 cells were co-transfected with a SEAP expression vector containing 24 tandem MCP-specific MS2-box repeats (reporter: PhCMV-SEAP-(MS2-box)24-HHR-pA, pSL...
Non-limiting examples of mammalian cells that can be used in accordance with the present invention include BALB/c mouse myeloma line (NSO/1, ECACC No: 85110503); human retinoblasts (PER,C6 (CruCell, Leiden, The Netherlands)); monkey kidney CVI line transformed by SV40 (COS-7, ATCC CRL 16...