Recombinant SV40-derived vectors have been used with pol II promoters to deliver transgenes that encode intracellular, cell membrane, and secreted proteins. They have also been used with pol III promoters to de
We made several changes to the mMoClo plasmids originally described33 in order to incorporate them into the workflow for our laboratory, in which many constructs are prototyped using pcDNA-based expression vectors. Details can be found in Supplementary Fig. 9, Supplementary Data 1, 2. We modified...
e Translational regulation by MCP-based STIF and other state-of-the-art regulation strategies. For STIF-mediated translation (red), HEK-293 cells were co-transfected with a SEAP expression vector containing 24 tandem MCP-specific MS2-box repeats (reporter: PhCMV-SEAP-(MS2-box)24-HHR-pA, pSL...
The episomal vectors of the invention promote high protein production in mammalian cells expressing the SV40 T Ag or Epstein-Barr ... CM Hsieh 被引量: 22发表: 2009年 Isolation of pcDNAI-based mammalian expression vectors from Escherichia coli strain MC1061/P3. Biotechniques. 1999 Mar;26(3)...
Each vector contains the neomycin/kanamycin-resistance gene under control of the prokaryotic β-lactamase promoter to provide kanamycin resistance in bacteria and the SV40 early promoter to provide G418 resistance in mammalian cells. pNTAP Expression Vectors The pNTAP expression vector is used to clone...
Southern, P. J., and Berg, P. (1982). Transformation of Mammalian Cells to Antibiotic Resistance with a Bacterial Gene Under Control of the SV40 Early Region Promoter. J. Molec. Appl. Gen. 1, 327-339. Wigler, M., Silverste...
Human embryonic kidney cell line 293 (HEK293) transformed with the SV40 large T antigen (HEK293T) were grown in Dulbecco’s modified Eagle Medium (DMEM; Invitrogen, cat. No. 21969-035) supplemented with 10% fetal bovine serum (ThermoFisher; cat. no. 10270106), 1% Penicilline/Streptomycin ...
(1982). Transformation of Mammalian Cells to Antibiotic Resistance with a Bacterial Gene Under Control of the SV40 Early Region Promoter. J. Molec. Appl. Gen. 1, 327-339. Wigler, M., Silverstein, S., Lee, L.-S., Pellicer...
The present disclosure can be taken to generally relate to the production of a polypeptide, such as a mammalian polypeptide. Provided is a vector suitable for expressing a respective polypeptide. In a first aspect there is provided an expression vector. The expression vector is a mammalian expressi...
We have observed that Py based expression vectors (plasmids containing Py origin of DNA replication) like SV40 based vectors can replicate several times within the cell and attain high copy number. In contrast to the COS cell expression system where plasmid replication overwhelms and eventually kills...