Training:Gibco Virtual Training Labs User Bulletin:Aseptic Techniques Checklist Video:Introduction to Cell Culture Video:Sterile Technique Video:Passaging Cells Video:Freezing Cells Video:Thawing Cells Video:AGT Dry Media Format in Your Large-Scale Manufacturing ...
For the generation of each single-cell sequencing library, 6 ovaries were collected from 3 different pups from a single pregnant female mouse. The ovaries were dissociated into single cells using 1 mg ml–1 Collagenase Type IV (Gibco; 17104019) at 37 °C for 30 min with pipetting ...
For the generation of each single-cell sequencing library, 6 ovaries were collected from 3 different pups from a single pregnant female mouse. The ovaries were dissociated into single cells using 1 mg ml–1 Collagenase Type IV (Gibco; 17104019) at 37 °C for 30 min with pipetting ...
使用Gibco 冻存培养基保护您的冷冻细胞 将细胞冻存可防止意外突变、衰老和污染。虽然未受保护的细胞冻存通常是致命的,但使用冻存培养基 冷冻细胞有助于保护细胞的长期活力,并最大限度地减少冷冻损伤,以便按计划提供完整的细胞。 查看细胞冻存和解冻方案,了解更多信息。 在DMSO 中冷冻细胞 使用时,冻...
Various gelatin-containing gel materials are used as scaffolds for animal and human cell culturing within the fields of cell technologies and tissue engineering. Cryostructuring is a promising technique for the preparation of efficient macroporous scaffolds in biomedical applications. In the current study...
Gibco™ Recovery™ 细胞培养冻存培养基是一种用于哺乳动物细胞培养的低温保存完全培养基。 保护您的细胞及研究结果: 从低温保存复融细胞后,细胞复苏率和活力增加 即用型冻存混合物——不需逐次用多种试剂自行配制冻存液 Ordering Information 货号产品名称规格单...
Equal amount of cell lysates was added to the Dynabeads Protein G coupled to the target antibody or the non-specific IgG as a control. The samples were further processed as above. For experiments in HEK293T cells, cells were maintained in DMEM medium (GIBCO), containing 10% FCS, penicillin...
Cell Lines and Culture Conditions Mouse ESCs were cultured in standard ES-DMEM: DMEM (GIBCO), 2 mM GlutaMax (GIBCO), 0.1 mM 2-mercaptoethanol (Sigma), 0.1 mM nonessential amino acids (GIBCO), 1 mM sodium pyruvate (GIBCO), 15% FBS (ESC tested, Hyclone catalog number SH30071.03), and ...
The filtered cells were mixed with 5 ml of serum containing neurobasal plus media (GIBCO) and centrifuged at 2,000 RPM at room temperature (RT) for 3 min. The supernatant was discarded, and cell pellet was washed twice with 1× PBS and with same settings of centrifugation. After washing,...
C at 500 × gfor 10 min and followed by immediate freezing of the cell pellet on dry ice. Samples were stored at −80 °C. During this study, we tried nine different approaches for the separation of brain tissue into single-cell suspension. The sample yield for each approach ...