In 1991, the catalytic site was characterized.221 The first structures (PDB codes 1XSI, 1XSJ, 1XSK) of a GH 31 α-glucosidase, YieI from Escherichia coli, were reported by Strynadka, Withers, and coworkers in 2005.222 Human intestinal maltase-glucoamylase, MGAM, (2QLY), a GH 31 ...
13 Deletions and nonsense mutations are associated with the infantile form, whereas “leaky” mutations, such as the common IVS1-13T > G splice-site mutation, are associated with the adult form.15 The lack of severe and systemic involvement of the adult and childhood forms might be explained...
Table V. Glycosidase activities of intestinal biopsy specimens from patients with congenital sucrase-isomaltase deficiency and from control subjects1 Subjects Age (years/ months) Biopsy site oucrase Isoni altase Enzymic activities units/g protein Total gluca- mylase Heat-resistant Maltase Glucamylase...
The results of molecular dynamics simulation analyses confirmed the docking data and showed deep penetration of L2 and L4 into the active site of MGAM. Based on cell cytotoxicity assessments, no significant cell death induction was observed. Hence, these functional MGAM inhibitors might be ...
The hydrophobicity of this tryptophan was thought to be critical for substrate binding and supporting catalytic action of the three carboxylate residues at the active site.doi:10.1016/j.ijbiomac.2015.02.035Rachadech, WanitchaNimpiboon, Pitchanan...
Domain B had a loop-rich structure containing one short helix and a small antiparallel β-sheet while the folding of domain C was atypical for GH13 enzymes consisting of five antiparallel β-strands in a double Greek-key motif. The active-site cleft was created by domains A and B forming ...