This invention is based, at least in part, on the discovery that a small-molecule inhibitor (JQ1) of the bromodomain and extra-terminal (BET) subfamily of epigenetic reader proteins is essential for chromatin remodeling during spermiogenesis. Biochemical analysis confirms that occupancy of the BRDT ...
After incubating in an incubator at 37 °C for 30 min with 5% CO2, the light absorbance was measured at 450 nm using a microplate reader (250058, BioTek, China). Quantitative real-time polymerase chain reaction (PCR) Total RNA was isolated by using a TRIzol™ kit (Invitrogen, Carlsbad,...
50%: 1 out of 2 females got pregnant, 0%: none of the 2 females got pregnant) found in Ctl (n = 5) and cKO mice (n = 6).GSpearman’s Rho was employed to assess the strength and direction of the relationship between the levels of ED obstruction (number of ...
(For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.) 4. Intra-occupational gender discrimination According to the result of Brown decomposition, the intra-occupational gender discrimination is the main cause of the gender...
(For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) 3.3. E2 strongly attenuates colitis-associated tumorigenesis in Nrf2 KO mice at week 16 The tumors that developed in the distal region of colon following AOM/DSS ...
Kinetics were measured at 650 nm using plate reader (Bio-Tek Synergy 2). Isolation of microglia from adult mouse brain Mice were anesthetized by Fatal Plus pentobarbital with i.p injection and perfused with 30 ml of ice-cold dPBS. Brains were minced well using razor blade in a petri ...
The OD of the samples was measured using a 96-well plate reader at 450 nm (iMark™ Microplate Absorbance Reader; Biorad, CA, USA). The amounts of acetyl histone H3 and H4 were measured by comparing to the kit-supplied standards. RNASeq of the amygdala and data analysis Total RNA ...
an educated guess based on our knowledge of the history of the region and settlement patterns. We hope that the reader takes “probably” or “likely” to mean just that, and not assume that sentences containing these phrases constitute “proof.” As a rule, we will only include information...
Body mass was measured at 12 and 16 weeks of age (males, n = 28 treatment, 30 control; females, n = 16/group). At 12 weeks of age, DXA scans were used to assess body composition in male and female mice. Measurements were obtained for (A) male body mass, (B) female ...
(Fig.5h). We next tested the expression levels of CCR5, a co-receptor of CCL5 and CCL4, by various Ly6C and 6G subsets of TGFβ1+myeloid cells, including TNs. We found that CCR5 expression by aged TNs was comparable to that in young TNs (Suppl. Fig.7d), and that CCR5 expression...