Single-labeled DAB-stained cells for pERK were counted in the nucleus accumbens (NAc) core and shell subregions, the caudate putamen (CPu), basolateral amygdala (BLA), lateral amygdala (LA), central amygdala (CeA), the OFC, the anterior cingulate area (ACA), prelimbic (PL), and infralimbic...
we examined the reproductive morphology of wild-type andTug1−/−male mice. Testicular descent appeared normal, and we did not observe any other gross morphological abnormalities in their reproductive system upon dissection (Additional file4: Fig. S4B). We measured testis mass relative to total ...
Illustrations of gross dissections of the penis and perineal muscles in a male mouse (A), Damaraland mole-rat (B), and silvery mole-rat (C).Marianne L. SeneyDiane A. KellyBruce D. GoldmanRadim ŠumberaNancy G. Forger
Dissection of dCA1 and vCA1 subregions from the hippocampus We employed a vibratome (Campden 7000 smz, UK) to slice the mouse brains in ice-cold artificial cerebrospinal fluid. Subsequently, for dCA1, brain slices ranging from AP −1.46 mm to AP −2.46 mm were collected, and the ...
Dissected hippocampi were immediately frozen in liquid nitrogen after dissection. According to the manufacturer's instructions, rat ELISA kits (Shanghai Mlbio Technology, China) were used to determine the levels of IL-1β and TNF-α. 2.11. TEM Rats were anesthetized (1 % sodium pentobarbital 50...
Jamaican fruit bats (Artibeus jamaicensis) naturally harbor a wide range of viruses of human relevance. These infections are typically mild in bats, suggesting unique features of their immune system. To better understand the immune response to viral infe
Liver from each rat was removed after dissection and preserved in 10% formalin. Then representative blocks of liver tissues from each lobe were taken and possessed for paraffin embedding using the standard microtechnique (Galighor and Kozloff, 1971). Sections (5 μm) of livers stained with hemot...
Ketamine produces rapid antidepressant effects at sub-anesthetic dosage through early and sustained activation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs), however, the exact molecular mechanism still remains unclear. Tran
Corpus callosum dissection for single-nucleus RNA-seq Brain samples were collected from a total of 16 male C57BL/6 J mice (4 mice per group). After perfusion with 1% PBS, the brains were promptly placed in a coronal stainless steel brain matrix that had been pre-chilled on ice. Clean...
due to the difficulty its dissection represents. Defining a metric to measure the distance between fibers allows the application of clustering methods partitioning the fibers into clusters based on the computation of connectivity matrices that provide for any couple of fibers a measure of the affinity...