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not only practical but also hygienic, preventing your dog from being harassed during their heat cycle or while potty training. The durable and waterproof material ensures that your dog can wear the diaper comfortably in any season, from autumn to winter, without compromising on style or ...
The role of As and FIS on the activities of antioxidative enzymes and oxidative stress markers is shown in Table 2. Arsenic treatment significantly (p < 0.05) lessened the activity of enzymes, namely CAT, SOD, GPx, GSR, and GSH level, whereas raised the level of oxidative stress marker...
The primers for the sequences of Luciferase and ChIP-PCR assay are: Pfkl site1, forward 5′-GGGGTACCGCCTGGGGAACCAGGGTTCC-3′ and reverse 5′-CCAAGCTTGTACCCGGTTTGTCCCGCCC-3′; Pfkl site2, forward 5′-GGGGTACCACAGGCAGGCGCACGGGGCGG-3′ and reverse 5′-CCAAGCTTCACCTTGCGCATCACCGCCG...
On the contrary, the mRNA expression levels of testicular Kiss1 were upregulated while Kiss1R were downregulated. The pituitary mRNA expression levels of FSH尾 and LH尾 decreased in the HU group. The levels of the antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione ...
The USP16 promoter: luciferase reporter plasmid was constructed in the pGL3 luciferase vector according to the manufacturer’s instructions (OBiO Technology, Shanghai, China). The motif in USP16-WT (GCTCATGAGTCAGCC) is mutated to USP16 Mutant (GCTCACAATCCACTT). HEK293T cells or HepG2 cells...
Quantitative RT-PCR was performed using SsoAdvanced SYBR Green Supermix (Bio-Rad) and a Bio-Rad iCycler IQ real time PCR system with indicated 20 μM primers (Integrated DNA Technologies, Coralville, IA) for interleukin-6 (IL-6; forward: CTGCCAGAGACTTCCATCCAGTT, reverse: GAAGTAGGGAAGG...
The homogenates were centrifuged (5 min, 1500g, 4 °C), and GSH and GSSG were measured in the supernatant using the Cuvette Assay kit for GSH/GSSG (Cat. No. GT35; Oxford Biomedical Research, MI, USA) following the manufacturer's instructions. Vitamin E was extracted from homogenized ...
The intensity and direction of correlations are indicated on the right side of the heatmap (red, positively correlated; green, negatively correlated). g Analogous scatter plots for the green module. The gene significance for a tumor (y-axis) is strongly correlated with module membership in the...
Primers used for genotyping: SOM-cre (5′-TCT GAA AGA CTT GCG TTT GG-3′, 5′-TGG TTT GTC CAA ACT CAT CAA-3′ and 5′-GGG CCA GGA GTT AAG GAA GA-3′), PV-cre (5′-GCT CAG AGC CTC CAT TCC CT-3′, 5′-AGT ACC AAG CAG GCA GGA GAT ATC G-3′ and 5′-CAG CCT ...