The present invention relates to the isolation of DNA regulatory regions of the malate synthase 1 gene (MLS1) from the yeast, The present invention further relates to the expression of heterologous proteins which have been introduced into a host cell and can be expressed under the regulation of...
The malate synthase gene, MLS1, of the yeast Saccharomyces cerevisiae is transcriptionally regulated by the carbon source in the growth medium. A MLS1-lacZ fusion gene, expressed at a basal level in the presence of 2% glucose, is derepressed more than 100-fold under conditions of sugar limita...
malate synthase (MLS, EC 2.3.3.9), which synthesizes malate from glyoxylate and acetyl-CoA1,2. In Arabidopsis seedlings, the transcript level and enzyme activity of ICL and MLS are induced after imbibition and decrease after post-germinative growth. This expression pattern is strongly correlated ...
malate synthase (MLS, EC 2.3.3.9), which synthesizes malate from glyoxylate and acetyl-CoA1,2. In Arabidopsis seedlings, the transcript level and enzyme activity of ICL and MLS are induced after imbibition and decrease after post-germinative growth. This expression pattern is strongly correlated ...
Therefore, malate synthase (MLS) in glyoxylate cycle has become a new drug target, particularly for thelatent infection. The focus of this study was to isolate new malate synthase inhibitors from a strain of actinomycetes, H7763. The fractions were obtained from the bioactivecrude acetone extract...
Constitutive and carbon source-responsive promoter elements are involved in the regulated expression of the Saccharomyces cerevisiae malate synthase gene MLS1 The malate synthase gene, MLS1 , of the yeast Saccharomyces cerevisiae is transcriptionally regulated by the carbon source in the growth medium. ...
Malate SynthaseFungal ProteinsCloning, MolecularSequence Analysis, DNAPlant DiseasesGene Expression Regulation, FungalA gene encoding malate synthase, a key enzyme of the glyoxylate cycle, has been cloned and characterized in the necrotrophic wheat pathogen Stagonospora nodorum . Expression studies of Mls1...
The present invention relates to the isolation of DNA regulatory regions of the malate synthase 1 gene (MLS1) from the yeast, host cell and can be expressed under the regulation of the MLS1 5′ regulatory promoter and MLS1 3′regulatory terminator regions. The MLS1 promoter is repressed in...
The present invention relates to the isolation of DNA regulatory regions of the malate synthase 1 gene (MLS1) from the yeast, Pichia pastoris. The present invention further relates to the expression of heterologous proteins which have been introduced into a P. pastoris host cell and can be ...
Zambuzzi-Carvalho PF, Cruz AHS, Silva LKS, Goes A, Soares CMA, Pereira M: The malate synthase of Paracoccidioides brasiliensis Pb01 is required in the glyoxylate cycle and in the allantoin degradation pathway. Med Mycol 2009, 31:1-11....