An EMS mutant of the ZmMM1 gene in the B73 background (named zmmm1-1) was found in a B73 EMS library (maize EMS-induced mutant database, MEMD). This mutant contains a single nonsense mutation in the second exon, generating a stop codon and early termination of the ZmMM1 protein (Figu...
All the gene-indexed point mutations have been deposited on the MEMD website (Maize EMS-induced Mutant Database; http://www.elabcaas.cn/memd/) and the mutant seeds are available upon request. Prediction of the Effects of the CG>TA Mutations on Gene Function To assess the potential effects...
EMS-induced mutantChloroplast developmentDifferentially expressed genes (DEGs)KEGG enrichment analysisqRT–PCRChloroplast development together with chlorophyll biosynthesis plays an important role in plant survival and growth. Here, EMS-induced chlorophyll-deficient mutants ( wtg , wts, and wta ) were ...
A mutant with a defective kernel phenotype was isolated from an ethylmethanesulfonate-induced maize B73 background population and was subsequently nameddek55–1. Thedek55–1kernels segregated from self-pollinated progeny ofdek55–1/+heterozygotes at a 1:3 mendelian ratio (Fig.1a, Additional file ...
The EMS mutant of maize ZmBT2a was purchased from the Institute of Biotechnology, Chinese Academy of Agricultural Sciences. Identification of ZmBT2a and ZmCUL3 genes in maize The DNA sequence and amino acid sequence of the maize ZmBT2a gene were obtained from the MaizeGDB database (https://...
. High, high exposure; Low, low exposure.l, Schematic diagram ofZmWIKand EMS-induced stop codon to generateZmWIKnull mutant.m,n, GLS resistance in the wild-typeZmWIKandZmWIKnull mutant (n = 132). Scale bar, 15 cm. Inf–
To genetically validate that mutation ofARFTF17was responsible for the mutant phenotype, we obtained a different EMS-generated B73 allele from the MEMD database (http://elabcaas.cn/memd/public/index.html#/)15, and designated itfka1-2. Thefka1-2allele also contains a C to T transition in...
parallelled by progress in data sciences and the development of novel breeding approaches utilizing genomic information. Herein, we report on past, current and future developments relevant for maize breeding with regard to (1) genome analysis, (2) germplasm diversity characterization and utilization, ...
Mutants of ZmDnaJ were obtained from the MEMD website (Maize EMS-indueed Mutant Database, http://maizeems.qlnu.edu.cn/) (Lu et al., 2018). Maize mutants were identified by sequencing comparison (TableS1), and pure positive seeds were obtained by multiplication and substitution for ...
An ethyl methanesulfonate (EMS) mutant of Zm00001d047799 (EMS4-0be358) was ordered from the Maize EMS induced Mutant Database (https://elabcaas.cn/memd/public/index.html#) [35], and was backcrossed once with B73 and then selfed once. The mutation site was genotyped by sequencing with...