Mucosal-associated invariant T (MAIT) cells typically express a TRAV1-2semi-invariant TCR伪 that enables recognition of bacterial, mycobacterial, and fungal riboflavin metabolites presented by MR1. MAIT cells are associated with immune control of bacterial and mycobacterial infections in murine models....
Here we describe microbe-reactive MR1-restricted T cells that do not express TRAV1-2. Functional analysis reveals that these cells, although less prevalent than those that express TRAV1-2, can be found in PBMC from all individuals. Among MR1-Ag tetramer-positive cells, 1–4% are TRAV1-2...
As known MAIT antigens are derived from riboflavin metabolites, this suggests that TRAV12-2 þ clone recognizes unique antigens. Thus, MR1-restricted T cells can discriminate between microbes in a TCR-dependent manner. We postulate that additional MR1-restricted T-cell subsets may play a ...
Selected TCR sequences were produced as TCR molecules and subsequently used to detect MR1/5-OP-RU on the surface of antigen-presenting cells.ResultsThe majority (70%) of the MAIT cells express typical TCRs containing TRAV1-2 and TRAJ33 gene segments. The remaining MR1/5-OP-RU-reactive ...