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Moreover, the instrumental analysis was performed using liquid chromatography coupled with a triple quadrupole mass spectrometer (LC–MS/MS, Xevo TQS, Acquity H-Class, Waters, Milford, CT, USA). For the chromatographic separation, a CORTECS T3 column (100 mm × 2.1 mm, particle size 1.6 ...
Institutional review board approval (approval number: DEISC-PR-190122074; Hospital Regional Materno Infantil de Alta Especialidad) was obtained, and the donors provided written informed consent before participating in the study. A total of 990 mL of HBM was collected, mixed at Centro de ...
The total number of BAL cells was counted after BALF was centrifuged at 300× g for 10 min at 4 °C. For further cytokine analyses, BALF samples were frozen at −80 °C. Differential cells were allowed to adhere to a slide via cytocentrifugation (Cytospin; Thermo Shandon, Pittsburgh, ...