The blot(s) were incubated in primary antibodies: anti-actin (Santa Cruz), anti-Flag polyclonal (Sigma), overnight at 4 degrees. The blot(s) were washed in 1X TBS and then incubated in secondary antibody at room temperature, then developed using ECF (for anti-rabbit-AP or anti-mouse-...
buffer: 39 mmol/L glycine, 48 mmol/L Tris base, 0.037% SDS (electrophoresis grade), and 20% methanol. The membranes were incubated overnight with a primary polyclonal antibody for the detection of a specific protein: an...