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aDetection of HP-NAP by anti-FLAG M2 antibody using recombinant HP-NAP-based ELISA. Recombinant HP-NAP was purified by a small-scale DEAE Sephadex negative mode batch chromatography. Purified HP-NAP as an antigen was coated on an ELISA plate as described in “Methods.” The anti-FLAG M2 a...