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PBMCs were adsorbed onto CELLSTAR 96-well black flat-bottom tissue culture plates in Macrophage-SFM media (Thermo Fisher Scientific) supplemented with 7.5% autologous heat-inactivated (0.22 μm-filtered) serum for 90 min at 37°C. Non-adherent cells were removed by washing twice with PBS, and...
Still he had normal blood pressure good peripheral perfusion no signs of shock. He was diagnosed as potentially fatal macrophage activation syndrome.Sahbaa Fehr Mohamed HafezResearch and Reviews
On day 3, macrophage fusion was induced with IL-4 as above except that 10% heat-treated (1 hour × 56°C) autologous serum was used to supplement macrophage-SFM. On day 7, the cultures were washed with prewarmed PBS++ and fixed and permeabilized for 2 minutes with acetone at −20°...
doi:10.1038/s41598-018-30787-xRuiz-Alcaraz Antonio JoséCarmona-Martínez VioletaTristán-Manzano MaríaMachado-Linde FranciscoSánchez-Ferrer María LuisaGarcía-Pe?arrubia PilarMartínez-Esparza MaríaScientific Reports
Human Umbilical vein endothelial cells (HUVECs) were grown on Hu Fibronectin (10 μg/ml) coated plates at a constant seeding density of 2.5 × 103 cells/cm2 in Hu Endothelial SFM (GIBCO) supplemented with 1% FBS (HyClone, Australian), 40 μg/ml Endothelial growth ...
Student's t-test *P = 9 × 10−5 (OxLDL) and *P = 3 × 10−6 (SFM). Data are representative of five independent experiments using separate donors. Since iron-loading may promote oxidant stress, we used multiple complementary readouts to assess whether HA-mac differentiation modulates ...
(treated with 2.7 mM EDTA) from normal volunteers was centrifuged on Lymphoprep separation medium (Nycomed AS, Oslo, Norway), and monocytes were purified by adherence to Falcon 24-well plastic tissue culture plates (Becton Dickinson UK Ltd., Cowley, Oxford, UK) in Gibco Macrophage SFM (serum-...