However, all three chosen protocols which use Vit D3, IL-13/IL-4, and IL-4, respectively, failed to promote the polarization of macrophages with a reliable M2 phenotype. Therefore, optimization or development of a new M2 polarization protocol is needed to achieve macrophages with a reliable ...
Validation of 3 new miRNAs in macrophage polarization. (A–C) qRT-PCR validation for the expression of the three miRNAs miR-1931 (A), miR-3473e (B), miR-5128 (C), using primary BMDM polarized to the M1- or M2- phenotypes after 1, 2, 4, or 8 hours. (D,E) BMDM transfected ...
Stat6 is known to drive macrophage M2 polarization. However, how macrophage polarization is fine-tuned by Stat6 is poorly understood. Here, we find that Lys383 of Stat6 is acetylated by the acetyltransferase CREB-binding protein (CBP) during macrophage a
ObjectiveTo investigate the effects of apoptotic bodies (ABs) derived from dental pulp stem cells (DPSCs) on macrophage polarization and inflammation responsein vivo.MethodsHuman DPSCs were extracted, cultured and identified. Staurosporine was used to apoptosis induction and differential methods were perfor...
Scheme 1. Illustration of how electric microenvironment promotes osteogenesis in diabetes mellitus by modulating macrophage polarization. After corona poling treatment, the surface of the ferroelectric nanocomposite membrane displays induced charges. Within the electrical microenvironment, macrophages exhibit less...
Due to the role of PPARγ in macrophage polarization and anti-inflammation, PPARγ ligands have been used to treat metabolism-related inflammation and have shown significant anti-inflammatory therapeutic activity. Full agonist of PPARγ refers to a ligand which can bind to LBD domain with high-...
Macrophage polarization tumor-associated macrophages as a paradigm for polarized M2 mononuclear phagocytes 热度: m0 m1 m2 m3 M0 M1 M2 M3基本概念和含义详解 热度: AcceptedManuscript M1toM2MacrophagePolarizationinHB-EGFTherapyforNEC JiaWei,M.D.,GailE.Besner,M.D. ...
The molecular pathways that underlie macrophage polarization are of intense interest due to the importance of this cell type in a wide array of pathologies, ranging from autoimmune disorders to cancer, and the desire to be able to modulate their polarization state. However, there are very few rep...
Despite the continued recruitment of monocytes and their differentiation into macrophages, the inflammatory environment in atherosclerotic plaques and that found in inflamed joints, influences cellular polarization. This aspect is typified by an impairment of macrophage protective functions in these tissues, ...
macrophage polarization, we compared the sizes of M1 and M2 nuclei to that of M0, a state before polarization. The polarization states were confirmed by expression of iNOS and Arginase-1, as well as their cellular morphology. In response to the M1 polarization signal LPS and IFN-γ, the ...