Metabolism is involved in biological processes and plays an important role in the polarization phenotype of immune cells [69]. To elucidate if metabolism determines the macrophage phenotypes or whether macrophage polarization phenotypes determine metabolic status, numerous efforts have been made to determine...
To investigate the differential expression of SPLUNC1 during macrophage polarization, PBMCs- or THP-1-derived Mφs were stimulated with LPS/IFN-γ or IL-4/IL-13 for M1 or M2 macrophage polarization. The identification of M1 and M2 macrophages was performed by determining macrophage markers, CD...
Super-enhancers (SEs) govern macrophage polarization and function. However, the mechanism underlying the signal-dependent latent SEs remodeling in macrophages remains largely undefined. Here we show that the epigenetic reader ZMYND8 forms liquid compartments with NF-κB/p65 to silence latent SEs and re...
Cytokines associated with macrophage polarization were analyzed using quantitative real-time polymerase chain reaction(qRT-PCR) and enzyme-linked immunosorbent assay(Elisa). The expression of macrophages in plaques was sequentially elevated in the NC, AS, and AS PD groups(P AS > NC. Our preliminary...
Macrophage Polarization assay Phagocytosis assay Monocyte killing assay Multiplex cytokine assay Test the effect of your immunotherapeutic compound on macrophage differentiation, within the tumour microenvironment or in response to inflammation and autoimmunity ...
Atherosclerosis Macrophage polarization Geniposide New Zealand rabbit MAPK signaling pathway 1. Introduction Atherosclerosis (AS) is a progressive arterial disease with chronic intimal inflammation [1,2]. AS plaques could gradually develop from stable to vulnerable, and may suddenly rupture; this is consid...
Microglia/macrophage polarization after experimental intracerebral hemorrhage. Transl Stroke Res. 2015;6(6):407-9.H. Zhao, T. Garton, R. F. Keep, Y. Hua, and G. Xi, "Microglia/ macrophage polarization after experimental intracerebral hemorrhage," Translational Stroke Research, vol. 6, no. 6...
HMOX-1, a M2 macrophage enzyme with anti-oxidant activities, plays a functional role in restoring and maintaining cell anti-oxidant capacity, which in turn enhances the M1 to M2 macrophage polarization via the suppression of inflammation and mediating the production of IL-10 (an M2 stimulus) (...
In the present study, we investigated the anti-inflammatory effects of JHGT on lipopolysaccharide- (LPS-) stimulated M1 macrophage polarization generated via the mitogen-activated protein kinases (MAPKs) pathway in RAW 264.7 mouse macrophages. The reducing power of JHGT was also investigated using ...
macrophage polarization, we compared the sizes of M1 and M2 nuclei to that of M0, a state before polarization. The polarization states were confirmed by expression of iNOS and Arginase-1, as well as their cellular morphology. In response to the M1 polarization signal LPS and IFN-γ, the ...