(PEG) 4000 (35% (w/v)), and 109 protoplasts are mixed and incubated at room temperature for 2min. The mixture is then diluted 10-fold in T69 medium supplemented with 0.5M xylose, 0.5% (w/v) BSA, 1mM CaCl2, and 4mgcholineml−1. The protoplasts are then centrifuged, washed, and ...
Empty CellConcentration of Protein (mg mL−1)Volume (μL)Mass (μg) Prestained markers of different lengthsb unknown 10 unknown Concentrated pool from DEAE 5 10 Concentrated FNR purified by adsorption 0.02 0.10 a To complete this table, use the concentrations of protein from the Bradford assay...
1000,聚乙二醇4000,聚乙二醇7000,聚乙二醇10000,聚乙二醇13000分子量对照品适量,加流动相溶解并稀释制成每lm l中约含2m g的溶液作为对照品溶液.称取样品适量,加流动相溶解并稀释制成每lm l中约含2m g的溶液作为供试品溶液.照分子排阻色谱法(通则0514)测定,采用适宜分离范围的凝胶色谱柱,以0. lmol/l硝酸钠...
(9) (10) Ph. 14 (11) (12) 1500 1500 S 1500 1450 / 1500 – 1540 3000 3000 S or P 3000 3000 –– 3350 3350 S or P 3350 3350 4000 4000 4000 4000 S or P 4000 4000 –– 6000 6000 S or P 6000 6000 –– 8000 8000 S or P 8000 8000 6000 – ...
ASNase amidohydrolase Lyophilized white crystalline powder, (225 IU/mg) Intravenous or intramuscular [107] ASNase from Dickeya chrysanthemi Lyophilized white powder (10,000 Units). Intravenous or intramuscular [108] Recombinant ASNase from Escherichia coli Lyophilized white powder, water-soluble (1000 ...