The presence or absence of m6Am in RNA affects mRNA stability, regulates transcription, and modulates pre-mRNA splicing. Nevertheless, its functions in the heart are poorly known. This review summarizes the current knowledge and gaps about m6Am modification and its regulators in cardiac biology. ...
in the human transcriptome Hanxiao Sun1,5, Kai Li1,2,3,5, Xiaoting Zhang1, Jun'e Liu1, Meiling Zhang1, Haowei Meng 1 & Chengqi Yi 1,3,4✉ N6,2′-O-dimethyladenosine (m6Am), a terminal modification adjacent to the mRNA cap, is a newly discovered reversible RNA modification. Ye...
Changes in half-life of mRNAs containing either m6Am or m6A in HEK293T cells transfected with either Flag vector (Ctrl) or FTO with an N-terminal nuclear export signal (NES–FTO) were determined by RNA-seq. m6Am mRNAs are generally long-lived (see Fig. 3a) and show reduced half-lives...
in the human transcriptome Hanxiao Sun1,5, Kai Li1,2,3,5, Xiaoting Zhang1, Jun'e Liu1, Meiling Zhang1, Haowei Meng 1 & Chengqi Yi 1,3,4✉ N6,2′-O-dimethyladenosine (m6Am), a terminal modification adjacent to the mRNA cap, is a newly discovered reversible RNA modification. Ye...
Chemical structure of the eukaryotic cap structures indicating location of the m6Am modification catalyzed by PCIF1 is shown. (F) Northern analyses of total RNA from WT and Pcif1 KO mutant mouse testis to detect U snRNAs. Signal from 5S rRNA is used as a loading control. The numbers ...
CLIP-Seq data demonstrated that CTBP2 bound to similar mRNAs as compared with PCIF1. We then used the m6Am-Seq method to profile the mRNA m6Am site at single-base resolution and found that mRNA of TET2, a well-known tumor suppressor, was a major target substrate of the PCIF1-CTBP2 ...
The RNA modification m6Am is catalyzed by phosphorylated C-terminal domain (CTD)-interacting factor 1, i.e., PCIF1 [5], a writer protein specific to the cap-related m6Am, and could be demethylated by the fat mass and obesity-associated protein, i.e., FTO [6], one of the m6A ...
2182570 and 91740112 to C.Y.; 81602513 to J.C.) and the Joint Laboratory of International Scientific and Technological Cooperation (Beijing Municipal Science and Technology Commission, Cooperative study of RNA modification detection technology and early diagnosis of disease). Author Contributions J.L....
Changes in half-life of mRNAs containing either m6Am or m6A in HEK293T cells transfected with either Flag vector (Ctrl) or FTO with an N-terminal nuclear export signal (NES–FTO) were determined by RNA-seq. m6Am mRNAs are generally long-lived (see Fig. 3a) and show reduced half-lives...