Note that we should rotate to Euclidean time \mathcal{L}\rightarrow -\mathcal{L}^E. However, we can keep the same superspace action (2.28). The \beta =0 case corresponds to pure D0 states, rather than D2-D0 bound states. There is also the \alpha ^0_0 contribution, which correspon...
After washing, tetramethylbenzidine was added (1 h), the reaction was terminated by application of 2 N HCl (1 h), and the ab- sorbance was measured at 450 nm (Molecular Devices Spectra Max), according to the standard curve. All RIA and EIA samples were measured in duplicates, in two ...
Data are presented as the median ± interquartile range or mean ± standard error of the mean (SEM) depending on data normality. Statisti- cal analyses were performed using one-way analysis of variance (ANOVA) post hoc Tukey's test, Kruskal–Wal- lis post hoc Dunn's test, ...
Max Rady College of Medicine, University of Manitoba, Winnipeg, Manitoba, R3E 0T6, Canada; 3Neuroscience Research Program, Kleysen Institute for Advanced Medicine, Health Sciences Centre, Winnipeg, Manitoba, R3E 3J7, Canada Abstract Transient receptor potential melastatin 2 (TRPM2) is a calcium (...
Stimulation of native P2Y11 in human M2 macrophages On day 6, fully differentiated M2 macrophages were har- vested, washed and seeded in 100 µl RPMI1640 supple- mented with 5% FBS, 1% GlutaMAX, 10 mM HEPES, 1 mM sodium pyruvate, 1% NEAA and 1% Pen/Strep in 96-well...
For kinetic analyses, Km and Vmax were calculated by using a non-linear curve fitting program, Graphpad Prism 4, based on the Michaelis–Menten equation whereas cata- lytic efficiency (kcat/Km) of the purified esterase were cal- culated by using the values of Km, Vmax and the ...