M1/M2 macrophage polarizationPDMStopographyMesenchymal stem cells and macrophages (MQ) are two very important cells involved in the normal wound healing process. It is well understood that topological cues and mechanical factors can lead to different responses in stem cells and MQ by influencing their...
M2 macrophage polarization can be induced by different stimuli: IL-4 and/or IL-13, immune complexes and toll-like receptor, IL-1 receptor ligands or IL-10 [6]. Alternatively activated macrophages polarized by IL-4 and IL-13 are characterized by a limited production of pro-inflammatory ...
However, its function in regulating the innate immune response and microglial/macrophage M1/M2 polarization in TBI has not been addressed. The present study aimed to evaluate the role of Mer in regulating microglial/macrophage M1/M2 polarization and neuroinflammation following TBI. Methods The ...
Thus, by using genes that were differentially expressed in M1 and M2 macrophage-stimulated A549 cells, we sought to identify the M1/M2 gene signatures to predict the clinical outcomes of NSCLC patients. Three gene signatures were identified based on the patient cohorts that had been published ...
M1 type macrophage polarization model that was subjected to ATRA (30 µg/mL ) treatment for 24 h. The Annexin V -PE/7-AAD double staining method was used to detect the effect of ATRA on macrophage apoptosis,flow...
M2 macrophage activation can be divided into M2a, M2b, and M2c, and these activation states can be considered as microglia (Boche et al., 2013). The M2a activation state is stimulated by IL-4 or IL-13 and, is involved in phagocytosis and tissue restore. fIL-4 stimulates JAK1 or JAK...
As a chemokine, CX3CL1 can promotes M1 macrophage polarization, and inhibition of CX3CL1 can cause macrophages to transition from M1 to M2 [22]. As shown in Fig. 3A and B, a moderate concentration of CX3CL1 (5 ng/ml), as the final concentration for further experiments, was the opt...
Initially, the M1/M2 macrophage phenotype marker genes induced by IFNγand LPS (M1 inducer) were ana- lyzed using RT-PCR analysis. Treatment with IFNγ/LPS significantly up-regulated the mRNA levels of the M1 marker gene iNOS or IL12 in Ana-1 and RAW264.7 macrophages (P ≤...
结论CD137信号可能通过调控巨噬细胞M1/M2极性转变促进血管新生。 关键词: 动脉粥样硬化;巨噬细胞极化;CD137;血管新生 ABSTRACT ObjectiveTo investigate whether CD137 signaling can promote angiogenesis via regulating macrophage M1/M2 polarization. Methods(1) The primary peritoneal macrophages in mice induced by...
M1 macrophages are considered to have a pro-inflammatory phenotype, while M2 macrophages have an anti-inflammatory phenotype and can secrete anti-inflammatory factors, induce progenitor cells and promote growth factor release. Promoting M2 macrophage phenotype polarization can effectively promote bone ...