The Endoplasmic Reticulum Is the Main Membrane Source for Biogenesis of the Lytic Vacuole in Arabidopsisdoi:10.1105/tpc.113.114827Vacuoles are multifunctional organelles essential for the sessile lifestyle of plants. Despite their central functions in cell growth, storage, and detoxification, knowledge ...
the cells appeared to be detaching and dying. The elongated nucleus phenotype was detectable within 2 hpi and increased to 6 hpi (Supplementary Fig.3e). To assess host cell membrane integrity and cell viability, infected fibroblasts
cells coincubated with 8 μM KL1 (positive control for membrane lysis), in which the nuclei of lysed cells were stained by propidium iodide (PI), and FITC-dextran signals showed vacuole excluded rather than homogenous distribution in the cytosol and concentrated distribution in the nucleoli (Fig...
24 h later, cells were fixed in 4% PFA for 20 min, and immunofluorescence staining (IFA) of the inner membrane complex (using αGAP45) was used to count the number of parasites per vacuole and the number of vacuoles per field. The number of vacuoles containing either 2, 4, 6, ...
Mitochondrial contacts have also been observed with the yeast vacuole32 and peroxisomes33. It is hypothesized that the IMC cisternae in Apicomplexa are of ER origin34 raising the possibility that com- plexes that tether the ER and mitochondria in other organisms may also tether the IMC and ...
For epithelial cells, this involves inhibiting vacuole acidification, promoting vacuolar escape, countering autophagy, and niche construction in the cytoplasm and within plasma membrane blebs. Paradoxically, ExoS and other P. aeruginosa T3SS effectors can also have antiphagocytic and cytotoxic activities. ...
Mitochondrial contacts have also been observed with the yeast vacuole32 and peroxisomes33. It is hypothesized that the IMC cisternae in Apicomplexa are of ER origin34 raising the possibility that com- plexes that tether the ER and mitochondria in other organisms may also tether the IMC and ...
Toxoplasma gondii is an obligate intracellular parasite that invades host cells, creating a parasitophorous vacuole where it communicates with the host cell cytosol through the parasitophorous vacuole membrane. The lytic cycle of the parasite starts with its exit from the host cell followed by ...
We recently demonstrated that the LC3 conjugation system (ATG7, ATG3, and ATG12–;ATG5-ATG16L1) is required to target LC3 and IFNG (interferon, gamma)-inducible GTPases to the parasitophorus vacuole membrane (PVM) of a...doi:10.1002/bies.201700231...
Typical for VN are prominent lead deposits in the large vacuoles. The development and functional significance of the large vacuole are discussed.doi:10.1111/j.1463-6395.1983.tb00786.xMaria ReuterInstitute of Biology, Åbo Akademi, FinlandJohn Wiley & Sons, Ltd (10.1111)Acta Zoologica...