包装规格:1 KIT 市场价:8310元 优惠价:立即咨询 溶酶体分离试剂盒 Lysosome Isolation Kit 基本信息 EC number【EC 号】 233-140-8 NACRES NA.32 General description【一般描述】 溶酶体是大多数原核和真核细胞中普遍存在的细胞器,含多种酸性水解酶,参与细胞中蛋白的降解。溶酶体还含脂肪酶、核酸酶和多糖酶,...
Sigma-Aldrich 货号 MAK405 包装型号 1 KIT, sufficient for 50 extractions 规格纯度 sufficient for 50 extractions 参考价格 5877.98元*本价格含增值税费 服务 √原厂保证 √包邮 √增值税票 数量 -+ QQ微信 添加到订购单查看订购单 产品名称: Lysosome Isolation Kit ...
公司名称: SIGMAALDRICH 联系电话: 800 558-9160 产品介绍: 备注:sufficient for 25 g (tissue), sufficient for 20 mL (packed cells), enrichment of lysosomes from tissues and packed cells 基本信息 中文名称: 中文同义词: 英文名称: LYSOSOME ISOLATION KIT 英文同义词: LYSOSOME ISOLATION KIT CAS号...
RAW264.7 cells infected with FITC-labeled H37Ra at an MOI of 10 were collected for lysosome isolation using a lysosome isolation kit (BestBio, Shanghai, China) according to the manufacturer’s instructions. The fluorescent intensity of H37Ra was measured using a CytoFLEX flow cytometer (Beckman...
CytoTox 96 Non-Radioactive Cytotoxicity Assay kit for LDH release assay was purchased from Promega. Collagenase type II and Propidium Iodide (PI) were purchased from Sigma. Antibodies were purchased as follows: LAMP1 (CST 9091S, clone D2D11 and DSHB H4A3), SNX2 (BD, 611308, clone 13), ...
PIERCE 89850 Pro-Ject (tm) Protein Transfection Reagent Kit KIT 4107 non stock PIERCE 89853 Phosphopeptide Isolation Kit KIT 4284 non stock 产品留言 标题 联系人 联系电话 内容 验证码 点击换一张 注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!2.如有必要,请您留下您的详细联系方式! 相关...
The clear supernatants were collected, and protein amounts were quantified using the BCA kit (Sigma-Aldrich). To perform co-immunoprecipitation, cells were lysed in ice-cold TAP lysis buffer (20 mM Tris pH 8.0, 150 mM NaCl, 0.5% NP-40, 1 mM MgCl2, 1 mM Na3VO4, 1 mM ...
Calcium content was measured by Calcium Assay Kit (Cayman Chemical, USA). The amount of calcium was normalized to the total amount of protein or the number of cells. 2.10. Exosome isolation Exosomes were harvested by following a modified Exosome isolation protocol [26]. Confluent VSMCs were ...
After another 24 hours, either 0.3 mmol/L bovine serum albumin (BSA) conjugated OA (Sigma-Aldrich) in medium or fatty acid free BSA (Sigma-Aldrich) in lipoprotein-deficient medium was added to the wells. After 4 hours, the media were collected for apoB measurement, and cells were lysed ...
Alzheimer s disease (AD) is characterized by cerebral deposition of β-amyloid peptide (Aβ). Aβ is produced by sequential cleavage of the Amyloid Precursor Protein (APP) by β- and γ-secretases. Many studies have demonstrated that the internalization