摘要:目的探索融合有锚定序列的EGFP蛋白,能否通过体外混合展示于乳酸乳球菌MG1363表面。方 法采用融合PCR方法扩增带有锚定序列的egfp(3LysM-egfp),亚克隆至pMD18T载体,测序正确后,插 入表达载体pET28a转化BL21(DE3)进行诱导表达、纯化,将纯化的3LysM-EGFP与乳酸乳球菌进行体外 ...
These eGFP positive cells are found in the C7–8 ipsilateral DRGs and C4–8 dorsal horn (DH) of the spinal cord post-SCI. Additionally, by utilizing anti-CD68 staining, resident macrophages (LysM-eGFP-/CD68+) can be differentiated from infiltrating macrophages (LysM-eGFP+/CD68+) and other ...